Words similar to tetrahymena
Example sentences for: tetrahymena
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Oxytricha anlage than in the Tetrahymena anlage, in that the density of broken ends would be, all else being equal, ~100× higher (0.
However, the ciliate literature is unclear about whether Tetrahymena telomerase might be able to act uncoupled from the action of the binder/cutter (see Introduction).
0. Of 675 species with at least 20 protein-coding sequences tabulated from nuclear DNA, we excluded 53 eukaryotes and 17 bacteria on the grounds that they had alternative genetic codes (for example, Tetrahymena and Mycoplasma ), or had introns accidentally tabulated in the database as part of the coding sequence (for example, Pongo and Homo ). These were detected as an excess of termination codons greater than 1 per 20 coding sequences (that is, at least 5% more stop codons than genes).
The electrophoresed samples were transferred onto ImmobilonP membranes (Millipore) by electroblotting for 1 hr in buffer containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, 10% methanol and 0.0025% Na dodecyl SO 4 . Prestained molecular mass markers (161-0372, BioRad) were supplemented with axonemal dynein from cilia of Tetrahymena (MM 528 kDa) in order to obtain a standard of sufficiently high molecular mass.
Tetrahymena telomerase and the binder/cutter appear to act concertedly in vivo , as if in a physical complex [ 48 49 ] . However, this contrasts with in vitro properties of the enzyme from exconjugants: 1.) it acts on non-telomeric primers [ 44 50 ] , and 2.) it apparently is no larger than the vegetative enzyme [ 44 ] . Furthermore, telomerase can act in the vegetative MAC of Paramecium , uncoupled from chromatid breakage [ 51 ] . It remains to be seen if a complex between telomerase and binder/cutter activity does exist.
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