Words similar to tetrahymena
Example sentences for: tetrahymena
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A short 15 bp cis -acting sequence, cbs, is necessary and sufficient to direct breakage at or near it in Tetrahymena , presumably being the binding site of the breakage enzyme - the "cutter" [ 34 35 36 ] . The cbs sits in eliminated sequence, a short, variable distance from the positions that receive telomeres.
0. Of 675 species with at least 20 protein-coding sequences tabulated from nuclear DNA, we excluded 53 eukaryotes and 17 bacteria on the grounds that they had alternative genetic codes (for example, Tetrahymena and Mycoplasma ), or had introns accidentally tabulated in the database as part of the coding sequence (for example, Pongo and Homo ). These were detected as an excess of termination codons greater than 1 per 20 coding sequences (that is, at least 5% more stop codons than genes).
5 ends/kbp in Oxytricha MIC DNA vs . 0.005 ends/kbp in Tetrahymena ).
Although chromatid breakage requires an endonuclease activity, the cutter, directed by a Cbs-binding activity, no Cbs-dependent "binder/cutter" activity has been detected in vitro . The cutter could be an activity of telomerase itself [ 39 43 ] . Telomerase of both Tetrahymena and Euplotes has a single-strand endonuclease activity [ 45 43 ] . It can trim potential primers back, so that the 3' terminus pairs with template RNA [ 46 ] . Thus, while it may serve a 3' editing function, the existence of a nuclease activity in potential proximity to the site of chromatid breakage is provocative, suggesting that telomerase provides the
Thus, in Tetrahymena and Paramecium telomeres are added at a heterogeneous collection of clustered sites, which we here refer to as "multi-TAS regions," whereas in E. crassus only one TAS is used for each MAC-destined end ("single TAS regions").
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