Words similar to tetrahymena
Example sentences for: tetrahymena
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The electrophoresed samples were transferred onto ImmobilonP membranes (Millipore) by electroblotting for 1 hr in buffer containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, 10% methanol and 0.0025% Na dodecyl SO 4 . Prestained molecular mass markers (161-0372, BioRad) were supplemented with axonemal dynein from cilia of Tetrahymena (MM 528 kDa) in order to obtain a standard of sufficiently high molecular mass.
Thus, in Tetrahymena and Paramecium telomeres are added at a heterogeneous collection of clustered sites, which we here refer to as "multi-TAS regions," whereas in E. crassus only one TAS is used for each MAC-destined end ("single TAS regions").
Tetrahymena telomerase and the binder/cutter appear to act concertedly in vivo , as if in a physical complex [ 48 49 ] . However, this contrasts with in vitro properties of the enzyme from exconjugants: 1.) it acts on non-telomeric primers [ 44 50 ] , and 2.) it apparently is no larger than the vegetative enzyme [ 44 ] . Furthermore, telomerase can act in the vegetative MAC of Paramecium , uncoupled from chromatid breakage [ 51 ] . It remains to be seen if a complex between telomerase and binder/cutter activity does exist.
0. Of 675 species with at least 20 protein-coding sequences tabulated from nuclear DNA, we excluded 53 eukaryotes and 17 bacteria on the grounds that they had alternative genetic codes (for example, Tetrahymena and Mycoplasma ), or had introns accidentally tabulated in the database as part of the coding sequence (for example, Pongo and Homo ). These were detected as an excess of termination codons greater than 1 per 20 coding sequences (that is, at least 5% more stop codons than genes).
The exact nucleotide positions where telomere repeats are added have been examined in several ciliates (reviewed by [ 23 53 ] ). Cloned chromosome ends from 81 MAC family indicated heterogeneity of TAS placement, which we attributed to exonuclease erosion of the broken ends prior to telomerase action [ 27 ] . Analogous multi-TAS regions have been described in Tetrahymena ( [ 49 ] ; E. Hamilton and E. Orias, pers.
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