Words similar to tet
Example sentences for: tet
How can you use “tet” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were plated onto glass coverslips in 6 well tissue culture plates in selective medium, induced the next day with 1 μg/ml Tet and fixed with 2% paraformaldehyde 24 hours later.
The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).
After IPTG induction (100 μM, 1 hour, 37°C) and plating on ampicillin (100 μg/ml) ± tetracycline (25 μg/ml) plates, mobility events were identified as amp R tet R colonies.
Rab24 contains a unique arginine-rich insert, 126EEDRRRRR 133 [ 25 ] in the domain cognate to loop-8 in H-Ras [ 34 ] . The latter sequence is reminiscent of unusual arginine-rich nuclear localization signals that allow proteins like HIV tet and rev to undergo nuclear translocation by association with importin-β, in the absence of importin-α [ 45 46 ] . Since the loop-8 insert in Rab24 lies adjacent to the conserved N(T)KxD nucleotide binding cassette, we hypothesized that conformational changes caused by the D123I substitution might render the Rab24 mutant capable of disrupting the nuclear import machinery [ 47 ] . To test this possibility, we co-expressed mycRab24wt or mycRab24(D123I) with GR-GFP, a fusion between the glucocorticoid receptor and green fluorescent protein.
The late Peter Braestrup did that in Big Story , which showed how badly the press had misreported the Tet Offensive in 1968 and repercussions it caused.
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