Words similar to tet

Example sentences for: tet

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The P1 primer was the same for the two OCPs and detection and specificity was achieved by two allele-specific Amplifluor P2s carrying either a FAM or a TET fluorophore.
For the G3C and G3U revertants, the donor plasmids from these tet R colonies were also isolated and sequenced to verify that they had the original mutations.
The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).
The two Amplifluors are labeled with a differently colored fluorophore: fluorescein (FAM) and tetrachloro-6-carboxyfluorescein (TET) and contain an internal nonfluorescent quencher DABCYL.
In this report, the ratio of amp R tet R to amp R colonies of each mutant, normalized to the wild-type ratio, was used as a measure of mobility frequency.