Words similar to terminated

Example sentences for: terminated

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Then, in October 1999, Sharif was deposed by General Pervez Musharraf, and the plan was terminated.
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
The ternary complex (E-DNA-dNTP) formation was assessed by incubating the binary complexes of enzyme and dideoxy terminated template primer in the presence of next correct dNTP [ 25 ] . The binary complexes were formed by incubating 10-50 nM of the wild type enzyme or its mutant derivatives with 0.3 nM of 5'- 32P-labeled dideoxy terminated 33-mer/21-mer template-primer as described above.
The labelling reaction was terminated by transferring the slides to 50 mL of stop buffer (10 mM EDTA, pH 8.0) for 5 minutes.
The reaction was allowed to continue for 3.5 to 4 hrs at 30°C and terminated by the addition of 1.3 ml of 100% TCA.