Words similar to terminated

Example sentences for: terminated

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Cells were washed twice in PBS and then incubated with Sulfo-NHS-LC-Biotin for 30 min at 4 o C. Biotinylation reactions were terminated by one wash in Tris followed by two washes in PBS.
Reactions were carried out at 37°C for 30 sec and 1 min and terminated by the addition of equal volume of Sanger's gel loading dye [ 35 ] . The cleavage products were analyzed on an 8% denaturing polyacrylamide-urea gel and scanned on a phosphorImager (Molecular Dynamics Inc.).
An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.
Thus, the rapid degradation of these mRNAs allows their levels to rapidly drop when transcriptional activation is terminated.
After 90 min at 30°C, the reaction was terminated by the addition 5 × SDS sample buffer.