Words similar to terminal
Example sentences for: terminal
How can you use “terminal” in a sentence? Here are some example sentences to help you improve your vocabulary:
When a patient's clinical status has reached a terminal disease progression stage (such as New York Heart Association class IV) and they are unable to perform exercise testing, as in Example 3, the exercise time should also be equal to zero seconds, and not simply regarded as missing data.
We used the Quickchange kit (Stratagene) to generate site directed mutants, V80R and A61D from wild-type TEL-SAM cloned into a modified pET3c vector (Novagen) [ 7 ] . The expressed protein sequence includes an MEKTR leader sequence, followed by residues 38-124 of the TEL protein and then a C-terminal His tag.
The three tetracysteine motifs do not form a stably folded structure on its own, but when combined with the 14 kDa C-terminal domain, forms a stably folded 268 amino acid DNA binding domain (ZD domain) that has higher affinity for single-stranded DNA than the 121 amino acid 14 kDa C-terminal region by itself [ 6 ] . Recent sequence and structural analysis suggests that the 14 kDa domain is evolutionarily related to the three tetracysteine motifs and belongs to the zinc ribbon family [ 7 ] . The ZD domain in E. coli topoisomerase I probably evolved from a domain that binds five Zn(II) originally.
Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) (In situ Cell Death Detection Kit, POD, Boehringer-Manheim).
The N-terminal part of the domain contains a universally conserved acidic residue that may play a critical role creating the local environment for NO selectivity.
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