Words similar to temperature-sensitive
Example sentences for: temperature-sensitive
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This prediction was then validated experimentally by showing that MJ1477 had CysRS activity in vitro and that an ortholog of MJ1477 from D. radiodurans, DR0705, complemented a CysRS deficient, temperature-sensitive, lethal E. coli mutant strain [ 18].
Moreover, the arrest of cdc5-1 , cdc5-2 , cdc5-4 , and cdc5-7 temperature-sensitive mutants in late mitosis was potently suppressed by the TAB6-1 allele (Park et al.
Murine podocytes immortalized with interferon-inducible temperature-sensitive SV-40 T antigen ( ts -SV-40 T) were isolated and infected with replication incompetent GFP-expressing HIV-1 virus, replication incompetent GFP-expressing single-gene mutants of HIV-1, or replication incompetent GFP-expressing control lentivirus containing GFP but no HIV-1 genes as previously described [ 7 8 ] . In all cases, 70% to 80% of podocytes showed GFP expression 4 days post infection (data not shown).
Loss of this enzymatic activity renders yeast cells temperature-sensitive for growth on galactose medium and hypersensitive to mild mutations in the yeast homolog of mammalian Cdk7, that is, Kin28, the kinase component of general transcription factor TFIIH [ 30].
A temperature-sensitive mutant of the yeast PP1 (Glc7), for example, exhibits a block in the M phase of the cell division cycle, and elevated expression of Sds22 suppresses this defect [ 38 ] . Structure-function analysis of recombinant mammalian PP1 has recently begun to map specific residues involved in interaction with other molecules.
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