Words similar to tel-sam

Example sentences for: tel-sam

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We used the Quickchange kit (Stratagene) to generate site directed mutants, V80R and A61D from wild-type TEL-SAM cloned into a modified pET3c vector (Novagen) [ 7 ] . The expressed protein sequence includes an MEKTR leader sequence, followed by residues 38-124 of the TEL protein and then a C-terminal His tag.
The structure of the V80E mutant TEL-SAM revealed a helical head-to-tail polymer in which the interface is made from two different surfaces on the protein.
We were, however, able to obtain a structure of a mutant TEL-SAM polymer, V80E [ 7 ] . The V80E mutation is in the center of the polymer interface and reduces the affinity of subunit association enough that the protein is relatively soluble above pH 7.0, where the Glu side chain is deprotonated.
In this report we have extracted a TEL-SAM dimer from the wild-type polymer and present its crystal structure.
The data was processed with DENZO/SCALEPACK [ 23 ] . The molecular replacement solution was found using AMORE [ 20 ] with a previously solved TEL-SAM mutant (V80E) dimer structure as the search model.