Words similar to tca

Example sentences for: tca

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This mechanism is familiar in nature, analogous steps being used in the TCA cycle, the ketoadipate pathway of lysine biosynthesis, and in leucine biosynthesis.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
24 cells with LY294002, a PI3K inhibitor, prior to the addition of TCA or TCDCA resulted in the loss of tolerance to these bile acids as demonstrated by the activation of caspase 8 (Figure 2B).
A portion of the supernatant was precipitated with TCA to estimate the level of total protein synthesis.
Moreover, the rates of decline in the amount of radiolabeled TCA associated with the cells after withdrawal of the radiolabeled bile acids from the culture medium was comparable among the different clones (Figure 9C), indicating that the rate of bile acid efflux from these cells was also not affected by the presence of HBAB.