Example sentences for: tca

How can you use “tca” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The beads were washed once with 70 μl of HEBD, and the wash was pooled with the supernatant, and precipitated with TCA (10% final) on ice for 30 min.

  • 24 cells with either TCA or TCDCA attenuated the GCDCA-mediated activation of caspase 8 (Figure 2C), suggesting that the activation of a PI3K-dependent survival pathway is dominant over the death pathway.

  • Insulin receptor was immunoprecipitated with specific antibodies, collected with protein A sepharose, and incubated with polyGlu 4 Tyr 1 peptides (Sigma, St. Louis, MO) in the presence of [γ- 32P]-ATP [ 14 ] . After completion of the kinase reaction, the mix was centrifuged and the phospho-peptide-containing supernatant precipitated with 20% TCA (w/v).

  • The marked morphological changes that occur during long term incubation with TCA [ 18 ] dissipate after the withdrawal of the bile acid from the culture medium, and the cells remain viable.

  • The primer pair for β-actin was GTC CTC TCC CAA GTC CAC ACA (forward) and CTG GTC TCA AGT CAG TGT ACA GGT AA (reverse), that of IL-8 was CTG CGC CAA CAC AGA AAT TA (forward) and ATT GCA TCT GGC AAC CCT AC (reverse), that of MCP-1 was GCC TCC AGC ATG AAA GTC TC (forward) and TAA AAC AGG GTG TCT GGG GA (reverse), that of IP-10 was CCA CGT GTT GAG ATC ATT GC (forward) and TGG AAG ATG GGA AAG GTG AG (reverse), that of RANTES was CGC TGT CAT CCT CAT TGC TA (forward) and GCT GTC TCG AAC TCC TGA CC (reverse), that of MIP-1α was TGC AAC CAG TTC TCT GCA TC (forward) and ACA GGG GAA CTC TCA GAG CA (reverse), and that of MIP-1β was CTG GGT CCA GGA GTA CGT GT (forward) and ACA GTG GAC CAT CCC CAT AG (reverse).


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