Words similar to target-specific
Example sentences for: target-specific
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Results are shown in Figure 4and Table 5. Since library construction was based on peptides previously selected for binding and selectivity to specific RNAs, the number of target-specific clones increased dramatically following in vitro maturation.
Upon ligation, the DNA circle creates an effective template for an exponential, or hyperbranching, RCA reaction [ 6 11 ] . The region of the OCP between the target-specific ends, which we have designated the OCP backbone, provides a binding site for a complementary primer (P1).
The 5' target-specific region of the OCP is 20-30 bp in length, while the 3' target-specific region is 12-20 bp [ 12 ] . The Tm of the 5' target specific region is ∼ 5°C above the ligation temperature, while that of the 3' target specific region is ∼ 15°C below the ligation temperature.
They are partly target- and partly backbone-specific, corresponding to the 3' target-specific region of the OCP, and 7-12 nucleotides of the adjacent backbone sequence.
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