Words similar to tailing

Example sentences for: tailing

How can you use “tailing” in a sentence? Here are some example sentences to help you improve your vocabulary:

For DNA fragments generated by ChIP (or blunt-end restriction) these limitations are effectively overcome by prior phosphatase treatment, by using recombinant terminal transferase enzyme, and by spiking a small quantity of terminating nucleotide into the tailing reaction.
If necessary, volume reduction of the eluate from the TdT tailing was performed in a vacuum centrifuge on medium heat.
Efficient tailing by terminal transferase requires the template DNA to be free of 3' phosphate groups, and is highly dependent on whether this template contains blunt- or overhanging-ends.
This protocol uses a terminal transferase tailing step and second strand synthesis to incorporate T7 promoters at the ends of the DNA fragments prior to IVT.
Second strand synthesis and incorporation of the T7 promoter sequence was carried out as follows: the 20 μL tailing reaction product was mixed with 0.6 μL of 25 μM T7-A 18 B primer (5'-GCATTAGCGGCCGCGAAATTAATACGACTCACTATAGGGAG(A) 18 [B], where B refers to C, G or T), 5 μL 10X EcoPol buffer (100 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 75 mM dTT), 2 μL 5.0 mM dNTPs, and 20.