Words similar to tailing
Example sentences for: tailing
How can you use “tailing” in a sentence? Here are some example sentences to help you improve your vocabulary:
For DNA fragments generated by ChIP (or blunt-end restriction) these limitations are effectively overcome by prior phosphatase treatment, by using recombinant terminal transferase enzyme, and by spiking a small quantity of terminating nucleotide into the tailing reaction.
The CFAS, however, found no evidence of any such tailing off in incidence, which also has implications for policy and planning.
If necessary, volume reduction of the eluate from the TdT tailing was performed in a vacuum centrifuge on medium heat.
Poly-dT tailing with terminal transferase
Second strand synthesis and incorporation of the T7 promoter sequence was carried out as follows: the 20 μL tailing reaction product was mixed with 0.6 μL of 25 μM T7-A 18 B primer (5'-GCATTAGCGGCCGCGAAATTAATACGACTCACTATAGGGAG(A) 18 [B], where B refers to C, G or T), 5 μL 10X EcoPol buffer (100 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 75 mM dTT), 2 μL 5.0 mM dNTPs, and 20.