Words similar to t-dna

Example sentences for: t-dna

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After we obtained several independent lines that grew on selection plates containing hygromycin, we used Southern blot analysis to determine the size of the LacOs and the relative copy number of the inserted T-DNAs in the transformed lines.
The L91 locus is a simple T-DNA locus at a defined chromosomal location.
The assay for the L91 locus was based on oligonucleotides specific for the L91 flanking sequence (AGGCACACAAGCCCAAAAAGAC-3') and the RB of the T-DNA [ 27 ] . PCR fragments representing the structure of the E82 locus were made as follows: Fragment 1 in Fig.
Most GFP-COP1 lines and the GUS-COP1 line L4 have been described [ 27 41 42 ] . Unsilenced GUS-COP1 and GFP-COP1 transgenes complement the cop1 mutation [ 42 43 ] . The C11 and C71 loci contain at least three and two linked T-DNAs, respectively, as seen by Southern blotting (not shown).
The trans-silencers as well as their targets reside in single-copy, gene-rich regions that are only sparsely populated with repetitive or transposable elements, features that have been shown to mediate epigenetic activity in other cases [ 18 31 32 ] . Therefore trans-silencing of GFP-COP1 is probably not mediated by transgene flanking sequences [ 27 ] . In contrast, as with other genes cited above, trans-silencing ability is correlated with transgene locus structure, given that the C73 and C97 trans-silencers contain multiple T-DNAs while their targets, E82 and L91, are essentially dimeric and monomeric, respectively.