Words similar to t-dna
Example sentences for: t-dna
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In contrast, none of four (single T-DNA) type L loci lost their endogene silencing ability (Fig.
Vice versa, this transition raises the question whether the originally strong transcription of the GFP-COP1 genes, or perhaps the ensuing PTGS phase, somehow sets the stage for the subsequent trans-silencing activity, especially if multimeric T-DNA loci are involved.
We speculate that a large T-DNA region (approximately 17 kb) and/or the presence of a large number of tandem repeats of LacOs in the T-DNA region may contribute to the low efficiency of gene transfer.
C73 contains at least three copies of a T-DNA comprising the 35S:GFP-COP1 gene as well as a kanamycin resistance gene driven by the nopaline synthase promoter ( nos:nptII ). The C73 locus is silenced transcriptionally (TGS) but retains partial expression of both nptII and GFP-COP1 . GFP-COP1 remains expressed in the seedling root but is largely silenced in the shoot.
The assay for the L91 locus was based on oligonucleotides specific for the L91 flanking sequence (AGGCACACAAGCCCAAAAAGAC-3') and the RB of the T-DNA [ 27 ] . PCR fragments representing the structure of the E82 locus were made as follows: Fragment 1 in Fig.
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