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How can you use “sα” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • When the 35 amino acid sequence was fused to the C-terminus of GFP (GFP- i2 35aa), this unique i2 sequence failed to direct GFP to intracellular membranes (Figure 5B); GFP- i2 35aa was diffusely distributed throughout the cytoplasm and nucleus, similar to what is observed for GFP alone (Figure 5A).

  • Thus, it is more likely that indeed i2 is deficient in binding to βγ, but this effect is a consequence of its instability.

  • Third, even though βγ can promote PM localization of i2 in the overexpression system described here, i2 remains less stable than α i2 (Figure 9), suggesting that such instability may also impair productive interactions with GPCRs.

  • Consistent with this idea, i2 was refractory to stable expression even though cells stably expressing α i2 were easily selected (not shown).

  • This is essential since suitable antibodies to detect and immuno-isolate i2 are not readily available.


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