Words similar to sα
Example sentences for: sα
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Myristoylation occurs co-translationally at the extreme N-terminal glycine, while palmitoylation occurs post-translationally at a cysteine immediately after the myristoylated glycine [ 15 ] . The subcellular site where palmitoylation occurs has not been well defined, although in one model palmitoylation occurs at the PM [ 16 ] . Cells expressing α i2 or sα i2 were metabolically labeled with 3H-palmitate or 3H-myristate.
When the 35 amino acid sequence was fused to the C-terminus of GFP (GFP-sα i2 35aa), this unique sα i2 sequence failed to direct GFP to intracellular membranes (Figure 5B); GFP-sα i2 35aa was diffusely distributed throughout the cytoplasm and nucleus, similar to what is observed for GFP alone (Figure 5A).
The inability to isolate cells stably expressing sα i2 can be attributed to its rapid degradation.
As a comparison, a non-palmitoylated mutant of α q , in which cysteines 9 and 10 have been substituted with serines, is found predominantly in the soluble fraction (Figure 2E, lanes 7 and 8), as described previously [ 13 ] . Surprisingly, in comparison to α i2 , sα i2 was always detected in fewer cells, by immunofluorescence analysis, and showed lower levels of protein expression, by western blot analysis of subcellular fractions (Figure 2E) or whole cell lysates (not shown).
Thus, the results of Figure 7are consistent with the proposal that the intracellular localization of sα i2 is due to its targeting to a degradative pathway, a pathway shared by α i2 .
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