Words similar to sα
Example sentences for: sα
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Not only is sα i2 targeted efficiently to the PM when co-expressed with βγ, but βγ co-expression increases overall levels of transiently expressed sα i2 and α i2 , as determined by immunoblotting (Figure 8G), and increases the number of cells expressing sα i2 , as observed by immunofluorescence microscopy (not shown).
A recent study identified a number of α5 helix residues in α t that when changed to alanines increased rates of guanine-nucleotide exchange ( i.e., decreased affinity for guanine-nucleotides) [ 5 ] . Individual mutation of amino acids in α t , cognate to T330, N332, V333, F337 in α i2 (Figure 1), increased nucleotide exchange rates [ 5 ] . Two of these critical amino acids, N332 and V333 in α i2 , are in fact the first two amino acids that are replaced by the novel splicing in sα i2 (Figure 1).
The C-terminal 35 amino acids of sα i2 , which replace the C-terminal 24 amino acids of α i2 (Figure 1), have been proposed to function as a Golgi targeting signal [ 3 4 ] . To test the potential importance of this C-terminal region in localization of sα i2 to intracellular membranes and prevention of sα i2 from localizing to PM, a deletion mutant of α i2 was constructed consisting of amino acids 1-331.
α i2 and sα i2 were immunoprecipitated from cell extracts, and incorporation of radio-label was analyzed by SDS-PAGE followed by fluorography (Figure 6A).
βγ prevents irreversible inactivation of α i1 , when measured in vitro at 37°C, and slows the inactivation of α i1 A326S [ 10 ] . Thus, βγ may stabilize sα i2 and α i2 A327S allowing βγ-dependent [ 25 26 27 28 29 ] PM localization.
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