Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
SYBR Green is a dye that fluoresces when bound to double-stranded DNA, thus signal is directly proportional to the amount of product formed during PCR amplification [ 18 23 ] . This method allows rapid and inexpensive comparison of gene expression across a large number of samples.
Each 25 μl PCR mix contained 7.875 μl Sybr ®Green Master Mix (1X Sybr ®Green Buffer, 3 mM MgCl 2 , 200 nM dNTP blend, 0.6275 U Amplitaq Gold ®, 0.25 U AmpErase ®UNG), 200-400 nM forward and reverse primers and approximately 5 ng template.
Direct comparison of SYBR Green RT-PCR results and Affymetrix results from the same RNA samples (n = 8 day 1-day 56 pairs) showed an overall qualitative agreement (i.e.
Standard reaction volume was 10 μl and contained 1X QuantiTect SYBR Green PCR master mix (Qiagen, Valencia, CA), 0.002 U AmpErase UNG enzyme (PE Biosystems, Forest City, CA), 0.7 μl of cDNA template and 100-500 nM of oligonucleotide primers.
The qPCR was performed on an Applied Biosystems PRISM 7700 Sequence Detection System using SYBR Green ®I chemistry.