Example sentences for: sybr

How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Quantitation and statistical analysis of SYBR Green RT-PCR data

  • SYBR Green RT-PCR was used to validate the microarray expression profiling data.

  • Heat dissociation curve analysis was performed after each SYBR Green run as a test of whether a single product had been generated in each PCR reaction; multiple peaks in the dissociation curves are indicative of multiple PCR products and thus reduced specificity and sensitivity.

  • Since nonspecific amplification was obtained in testing some of the primers sets with the -RT controls, optimization of the primer concentration was performed as per the SYBR green PCR protocol (Applied Biosystems).

  • Each 25 μl PCR mix contained 7.875 μl Sybr ®Green Master Mix (1X Sybr ®Green Buffer, 3 mM MgCl 2 , 200 nM dNTP blend, 0.6275 U Amplitaq Gold ®, 0.25 U AmpErase ®UNG), 200-400 nM forward and reverse primers and approximately 5 ng template.


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