Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
Quantitation and statistical analysis of SYBR Green RT-PCR data
SYBR Green RT-PCR was used to validate the microarray expression profiling data.
Heat dissociation curve analysis was performed after each SYBR Green run as a test of whether a single product had been generated in each PCR reaction; multiple peaks in the dissociation curves are indicative of multiple PCR products and thus reduced specificity and sensitivity.
Since nonspecific amplification was obtained in testing some of the primers sets with the -RT controls, optimization of the primer concentration was performed as per the SYBR green PCR protocol (Applied Biosystems).
Each 25 μl PCR mix contained 7.875 μl Sybr ®Green Master Mix (1X Sybr ®Green Buffer, 3 mM MgCl 2 , 200 nM dNTP blend, 0.6275 U Amplitaq Gold ®, 0.25 U AmpErase ®UNG), 200-400 nM forward and reverse primers and approximately 5 ng template.
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