Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
Individual genes observed to be significantly different by Affymetrix analysis and in both sets of SYBR Green RT-PCR experiments were screened as potential biomarker candidates.
A subset of the genes selected by the 5% LFC model were examined by RT-PCR using SYBR ®green dye (Molecular Probes) which interacts with double-stranded nucleotide sequences.
The PCR mix per reaction included 10 μl SYBR Green buffer, 1.5 mM MgCl 2 , 1 μM dNTP mix (with dUTP), 0.5 μl CMV primer mix, 2.5 units Ampli Taq gold, 0.05 units of uracil-N-glycosylase (UNG, Epicentre, Madison, WI) that had been diluted 1:10 in UNG buffer, and molecular grade water to bring the volume up to 45 μl with the addition of 5 μl of cDNA or standard.
SAHH and HSC70 were evaluated using the SYBR green reporter assay kit available from Qiagen (Valencia, CA).
Cycling parameters using SYBR green detection were 50°C for 30 min followed by 95°C for 15 min then 40 repetitive cycles at 95°C for 15 sec, 51°C for 30 sec, 72°C for 30 sec, and a fluorescent data acquisition step following a brief 15 sec incubation at 78.