Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
All real-time PCR reactions were performed in triplicate on an ABI Prism 7000 in SYBR Green Master Mix (Applied Biosystems), with denaturation at 94°C for 15 seconds, annealing at 55°C for one minute and extension at 60°C for one minute in a total of 40 cycles.
The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.
Individual genes observed to be significantly different by Affymetrix analysis and in both sets of SYBR Green RT-PCR experiments were screened as potential biomarker candidates.
PCR was performed using an iCycler iQ thermic cycler equipped with a real-time detection system (Bio-Rad, Hercules, California, United States) and a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, California, United States).
Cycling parameters using SYBR green detection were 50°C for 30 min followed by 95°C for 15 min then 40 repetitive cycles at 95°C for 15 sec, 51°C for 30 sec, 72°C for 30 sec, and a fluorescent data acquisition step following a brief 15 sec incubation at 78.
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