Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
All real-time PCR reactions were performed in triplicate on an ABI Prism 7000 in SYBR Green Master Mix (Applied Biosystems), with denaturation at 94°C for 15 seconds, annealing at 55°C for one minute and extension at 60°C for one minute in a total of 40 cycles.
Standard reaction volume was 10 μl and contained 1X QuantiTect SYBR Green PCR master mix (Qiagen, Valencia, CA), 0.002 U AmpErase UNG enzyme (PE Biosystems, Forest City, CA), 0.7 μl of cDNA template and 100-500 nM of oligonucleotide primers.
With the use of triplicate samples and no template controls, real-time PCR (iCycler™ iQ; Bio-Rad) with SYBR Green (Molecular Probes) as the detection method was performed to quantify the mRNA levels for selected breast tissues.
Quantitation and statistical analysis of SYBR Green RT-PCR data
A subset of the genes selected by the 5% LFC model were examined by RT-PCR using SYBR ®green dye (Molecular Probes) which interacts with double-stranded nucleotide sequences.