Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
Heat dissociation curve analysis was performed after each SYBR Green run as a test of whether a single product had been generated in each PCR reaction; multiple peaks in the dissociation curves are indicative of multiple PCR products and thus reduced specificity and sensitivity.
Standard reaction volume was 10 μl and contained 1X QuantiTect SYBR Green PCR master mix (Qiagen, Valencia, CA), 0.002 U AmpErase UNG enzyme (PE Biosystems, Forest City, CA), 0.7 μl of cDNA template and 100-500 nM of oligonucleotide primers.
To further confirm these transcripts as potential biomarkers of SU5416 administration, SYBR Green RT-PCR analysis was carried out in samples from a second Phase III trial using SU5416 (Trial B).
Since nonspecific amplification was obtained in testing some of the primers sets with the -RT controls, optimization of the primer concentration was performed as per the SYBR green PCR protocol (Applied Biosystems).
For individual reactions, 10 μl of each sample were combined with 15 μl of SYBR Green PCR Master Mix (Applied Biosystems) containing the appropriate primer pair at 350 nM.