Words similar to sybr
Example sentences for: sybr
How can you use “sybr” in a sentence? Here are some example sentences to help you improve your vocabulary:
A subset of the genes selected by the 5% LFC model were examined by RT-PCR using SYBR ®green dye (Molecular Probes) which interacts with double-stranded nucleotide sequences.
SYBR Green is a dye that fluoresces when bound to double-stranded DNA, thus signal is directly proportional to the amount of product formed during PCR amplification [ 18 23 ] . This method allows rapid and inexpensive comparison of gene expression across a large number of samples.
SAHH and HSC70 were evaluated using the SYBR green reporter assay kit available from Qiagen (Valencia, CA).
The PCR reactions were analyzed by electrophoresis using 1.5 % agarose gels, visualized with SYBR ®Gold nucleic acid staining (Molecular Probes; Eugene, OR), photographed, scanned and digitized with the UN-SCAN-IT™ gel automated digitizing system from Silk Scientific (Orem, UT).
Since nonspecific amplification was obtained in testing some of the primers sets with the -RT controls, optimization of the primer concentration was performed as per the SYBR green PCR protocol (Applied Biosystems).