Example sentences for: svec
How can you use “svec” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pCYIL-10 vector is an expression vector which was established by Dr. Xianmin Meng based on the pCY4B vector [ 40 ] . Cultured SVEC endothelial cells seeded in 24 wells were transfected at 70% confluency with SuperFect (Qiagen).
For NF-κB p65 blotting, SVEC were pretreated for 1 h with or without inhibitors, and then incubated for 1 h with either vehicle or TNF-α (20 ng/ml).
7 ± 5.4% as many of the TK-1 lymphocytes bound to SVEC compared to TNF-α stimulation (100%, or 'maximal' binding).
The TK-1 lymphocyte cell line used in this assay expresses high levels of the α4β7 integrin, [ 39 41 ] which can interact with multiple ligands including mucosal addressin-1 (MAdCAM-1), as well as VCAM-1, L-selectin and fibronectin [ 42 ] . In this system, TNF-α stimulated TK-1 adhesion to SVEC4-10 endothelial cells is at least 50% MAdCAM-1 dependent [ 41 ] . SVEC monolayers were grown in 48-well plates as described, and to activate endothelium, the monolayers were incubated with TNF-α (1 ng/ml) for 24 h.
Importantly, SVEC cells transfected with this vector showed no evidence of toxicity, altered proliferation and morphologically were indistinguishable from mock-transfected and untreated controls.
Loading...