Words similar to svec

Example sentences for: svec

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The SVEC4-10 line is an endothelial cell line derived by SV40 (strain 4A) transformation of murine small vessel endothelial cells, originally isolated from the axillary lymph node vessels of an adult male C3H/Hej mouse [ 37 38 ] . These cell types were all maintained in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal calf serum with 1% antibiotic/ antimycotic.
The pCYIL-10 vector is an expression vector which was established by Dr. Xianmin Meng based on the pCY4B vector [ 40 ] . Cultured SVEC endothelial cells seeded in 24 wells were transfected at 70% confluency with SuperFect (Qiagen).
There was no detectable human IL-10 signal in the non-transfected cell medium (control), nor any mouse IL-10 ( data not shown ). However, the medium from the IL-10 transfected SVEC medium showed a large and significant increase in the IL-10 concentration (1209 ± 2 pg/ml) at 48 h after IL-10 gene transfection (Figure 1).
Total cell RNA was extracted from SVEC cells using the RNeasy Kit (QIAGEN Inc., Valencia, CA) according to manufacturers instructions.
For NF-κB p65 blotting, SVEC were pretreated for 1 h with or without inhibitors, and then incubated for 1 h with either vehicle or TNF-α (20 ng/ml).