Words similar to supplemented
Example sentences for: supplemented
How can you use “supplemented” in a sentence? Here are some example sentences to help you improve your vocabulary:
The T cell lines HPB-ALL and CEM were maintained in complete media (RPMI-1640 supplemented with 10% FBS; 10 U/ml of both penicillin and streptomycin; and 1 mM L-Glutamine, all from Life Technologies, Grand Island, NY).
Following incubation with gefitinib or erlotinib, monolayers were washed twice with PBS (containing calcium and magnesium) and incubated with 7.5 μmol Calcein AM in supplemented RPMI-1640 (no serum) for 30 min.
To overcome this problem, we supplemented the random hexanucleotides that were used to prime the second RT reaction with a template-switch (TS) primer [ 25].
A full record of work in this field can be found in bibliographies compiled since 1948 under the names Sealock, Seely, and Powell (the latest edition being Bibliography of Place-Name Literature: United States and Canada, Third Edition , 1982), supplemented periodically in the pages of Names , the quarterly journal of the American Name Society.
Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.
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