Example sentences for: supplemented

How can you use “supplemented” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The T cell lines HPB-ALL and CEM were maintained in complete media (RPMI-1640 supplemented with 10% FBS; 10 U/ml of both penicillin and streptomycin; and 1 mM L-Glutamine, all from Life Technologies, Grand Island, NY).

  • Following incubation with gefitinib or erlotinib, monolayers were washed twice with PBS (containing calcium and magnesium) and incubated with 7.5 μmol Calcein AM in supplemented RPMI-1640 (no serum) for 30 min.

  • To overcome this problem, we supplemented the random hexanucleotides that were used to prime the second RT reaction with a template-switch (TS) primer [ 25].

  • A full record of work in this field can be found in bibliographies compiled since 1948 under the names Sealock, Seely, and Powell (the latest edition being Bibliography of Place-Name Literature: United States and Canada, Third Edition , 1982), supplemented periodically in the pages of Names , the quarterly journal of the American Name Society.

  • Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.


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