Words similar to supplemented

Example sentences for: supplemented

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Media was supplemented with glucose, sucrose, NaCl and ethanol in selected experiments as described in the Results.
Primary neuronal cultures of feotal rat forebrains were prepared as described previously [ 14 ] . Briefly, the forebrains of feotal rat pups (embryonic day 15) were isolated, and the cells were dispersed by repeated mechanical trituration in neuronal culture medium (NCM = Ham's F-12: Basal Medium Eagle, 1:1; supplemented with dextrose, 0.6 g/l; glutamine, 0.35%; and Pen-Strep, 1%).
The cells were then incubated in the same buffer supplemented with 50 μM neostigmine (to inhibit acetylcholinesterase) with or without 100 nM PMA for 45 minutes.
The electrophoresed samples were transferred onto ImmobilonP membranes (Millipore) by electroblotting for 1 hr in buffer containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, 10% methanol and 0.0025% Na dodecyl SO 4 . Prestained molecular mass markers (161-0372, BioRad) were supplemented with axonemal dynein from cilia of Tetrahymena (MM 528 kDa) in order to obtain a standard of sufficiently high molecular mass.
After 24 h, medium was replaced by culture medium supplemented with 0.5% FCS.