Words similar to supplemented
Example sentences for: supplemented
How can you use “supplemented” in a sentence? Here are some example sentences to help you improve your vocabulary:
Purified cells were cultured at 37°C in RPMI-1640 medium supplemented with 10% fetal bovine serum at a density of 5 × 10 6cells/ml and in the presence or absence of 10 nM PMA phorbol ester and or 1 mM dibutyryl cAMP + 1 mM phosphodiesterase inhibitor isobutylemthylxnthine (IMBX).
The medium was changed to serum-free medium (1% BSA/DMEM) supplemented with 50 μg/ml of ascorbic acid for 48 h.
Another large cell present after 1 week in the supplemented cultures had multiple nuclei (Fig.
16, was cultured in Dulbecco's modified Eagle medium (MEM; Life Technologies) supplemented with 10% defined fetal calf serum (Hyclone Laboratories) at 37°C in 5% CO 2 . Because phagocytosis of mycobacteria was shown to be enhanced with complement protein C3 [ 40 ] , macrophages were infected with M. paratuberculosis in the presence of 10% serum from a clinical cow with Johne's disease.
Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.