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Example sentences for: sunnyvale
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Ten micrograms of total RNA from each sample was used for RNase protection assay (mCK -2b and -3b Multi-Probe Template Sets, PharMingen, San Diego, CA, USA), exposed to a Phosphor screen, and quantified by a Phosphorimager with the use of Image Quant software (Molecular Dynamics, Sunnyvale, CA, USA).
Membranes were sequentially hybridized with radioactive probes for α2(I) procollagen and GAPDH and scanned with a PhosphorImager (Molecular Dynamics, Inc., Sunnyvale, CA) for mRNA quantitation.
2 (Molecular Dynamics, Sunnyvale, CA).
Oligonucleotides (50 μM, except where indicated otherwise) and PCR amplicons (100-200 nM) in 50% DMSO were printed onto SuperAmine slides (TeleChem International, Sunnyvale, CA) using an Intelligent Automation Systems (IAS) arrayer (Cambridge, MA) with a 12-pen print head [ 16].
The absorbance was measured at 410 nm using a microplate reader (Emax, Sunnyvale, CA).