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Example sentences for: suc
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Under repressing conditions (2% glucose), the level of SUC2 mRNA was increased by two-to-four fold in an nrg1Δ strain compared to a wild-type control (Figure 3A).
Deletion of MIG1, however, only partially relieves glucose repression at promoters such as SUC2, whereas deletion of either SSN6 or TUP1 completely abolishes glucose repression.
To test for glucose repression of SUC2 and GAL genes, 2-deoxyglucose was added to YP sucrose-antimycin A and YP galactose-antimycin A plates to a final concentration of 200 μg/ml [ 4].
These results strongly suggest that Nrg1, Mig1, and Mig2 are each required for glucose repression at the SUC2 locus, with Mig1 playing the major role.
To sensitize the screen, we used an allele of SUC2, SUC2-36, that allows an elevated level of SUC2 transcription in the absence of Swi/Snf [ 24].
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