Words similar to subnuclear
Example sentences for: subnuclear
How can you use “subnuclear” in a sentence? Here are some example sentences to help you improve your vocabulary:
To verify if the spots in nuclei correspond to the tagged loci, we examined whether the detected subnuclear localization of the expressed GFP-LacI/NLS fusion protein is sensitive to isopropyl-β-D-thiogalactoside (IPTG).
Under this condition, we found that we could obtain spatial information for subnuclear structures with excess unbound GFP-LacI/NLS fusion proteins.
More recently, evidence from fluorescence in situ hybridization (FISH) studies in different systems has suggested that various genomic loci can be found consistently in specific regions of the nucleus [ 4], and that subnuclear location can be correlated with gene activity [ 5].
AtCOP1 contains three conserved structural domains: a RING finger at the amino terminus, a coiled-coil domain in the middle, and a carboxyl-terminal WD40 repeat domain [ 9 10 ] . Each of the three conserved domains has been shown to mediate protein-protein interactions [ 11 12 13 ] . The subcellular localization of AtCOP1 is regulated by light in a tissue specific manner [ 14 15 ] . The hypocotyl cell nuclei contain high levels of COP1 in the dark and reduced levels in the light, suggesting that the nucleocytoplasmic partitioning of AtCOP1 is adjusted by a light-responsive mechanism [ 14 16 ] . The activity of AtCOP1 is at least in part regulated by its subcellular localization, as the degradation of HY5 is dependent upon the nuclear accumulation of AtCOP1 in the dark [ 4 ] . AtCOP1 was demonstrated to carry a single, bipartite nuclear localization signal located between the coiled-coil domain and the WD-40 domain (amino acid 294-314) and a cytoplasmic localization signal, which was mapped to a region partially overlapping with the RING finger and the coiled-coil domain (amino acid 67-117) [ 17 ] . Strikingly, AtCOP1 protein forms characteristic nuclear speckles when transiently expressed in onion epidermal cells or stably expressed in transgenic Arabidopsis [ 6 18 ] . The functional role of these speckles is currently unknown; however, a subnuclear localization signal consisting of 58 residues (amino acid 120-177) is required for their formation [ 19 ] .
In future, by marking individual cells with visual tags it should be possible to follow changes in nuclear shape as well as relative subnuclear position of the nucleolus and specific tagged genomic loci over long times-scales of the order of weeks.
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