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Example sentences for: subdomains
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The sequence identity within the HisRS domain of IfkA/B and yeast GCN2 is 28%, with strong conservation within the M1, M2, and M3 motifs that are characteristic of class II aminoacyl-tRNA synthetases [ 37 ] . The similarity between the kinase domains is much higher, with IfkA/B possessing 44% identity with yeast GCN2, with high conservation of all of the kinase subdomains.
It has been suggested that the stability of the dimer is related to the buried surface area between the two subunits [ 11 12 ] . In the nevirapine-bound HIV-1 RT crystal structure, the total contact surface area between the subunits is approximately ~4600 Å 2. The two major contact regions between the subunits which provide it stability are their connection subdomains and the thumb of p51 and RNase H domain of p66.
The blasticidin resistance cassette replaced a 391 bp fragment that includes the first four subdomains (the ATP binding site) of the kinase domain.
To this end, two peptides were synthesized and purified based on selected sequences in the subdomains of human nmt55/p54 nrb(Figure 1A).
Despite the fact that p51 shares an identical amino acid sequence with the N-terminal portion of p66, the two subunits assume different global folding patterns in the formation of the asymmetric heterodimer [ 9 ] . Structural determination through X-ray crystallography has revealed that the p66 subunit of HIV-1RT has its polymerase domain in an "open" conformation, with its subdomains forming a large cleft which accommodates DNA.
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