Example sentences for: subculturing

How can you use “subculturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After 2-4 days of subculturing, the cells (approximately 50% of confluency) were cultured in the medium supplemented with 0.3 μg/ml nocodazole (Sigma), a microtubule polymerization inhibitor, for 10 h to induce G2/M phase arrest.

  • The cell population was then expanded by sequentially subculturing into 25-cm 2tissue culture flasks, then to 75-cm 2tissue culture flasks.

  • Immortalized rat peritoneal mesothelial cells (Fred-Pe) developed in-house were grown in mesothelial cell culture media as previously described [ 12] for several months before experiment with weekly subculturing.


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