Words similar to stratagene

Example sentences for: stratagene

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Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.
Gb3 concentrations were calculated by densitometry (Eagle Eye II, Stratagene, LaJolla, CA) and standardized to total protein.
Desiccated brain tissue from adult Drosophila melanogaster was used to construct a library using the Stratagene Hybrid-Zap system.
DNA probes were 32P-labeled using the Prime-It II DNA labeling kit (Stratagene).
Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.