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Example sentences for: stratagene
How can you use “stratagene” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR amplification was performed in a 50 ul volume with the PfuTurbo Hotstart DNA Polymerase system (Stratagene) and included 0.2 mM dNTPs, 100 ng each primer, 2.5 units PfuTurbo DNA polymerase, 100 ng genomic DNA, and 1X cloned Pfu DNA polymerase reaction buffer.
Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.
Mutations of the opossum alpha-2C adrenergic receptor cDNA in pRc/CMV vector described previously [ 21 ] was accomplished using the QuickChange™ Site Directed Mutagenesis Kit (Stratagene, LaJolla, CA).
For first strand cDNA synthesis, 3 μg (unless otherwise specified) tumor total RNA, Universal Human Reference total RNA (Stratagene ®), or 150 ng poly(A) +RNA was mixed with 1 μg Eberwine primer (Operon ®) in RNase-free water to a total volume of 9 μl.
Oligonucleotide primers for the PCR reaction were based on the published sequence of murine Rab24 [ 24 ] . Kpn1 and BamH1 restriction sites were added to the 5' and 3' primers respectively, to facilitate cloning of the PCR product in frame with a 5' sequence cassette encoding a myc epitope (EQKLISEEDL), previously introduced into the pCMV5 expression vector [ 78 ] . The Rab24 cDNA was altered by overlap-extension PCR [ 79 ] , using appropriate mutator oligonucleotides and Pfu DNA polymerase (Stratagene Inc., La Jolla, CA), so as to introduce amino acid substitutions at D123 (D123I), T120 (T120A), S22 (S22N), S67 (S67Q), and a deletion of residues 127 through 130 (ΔEDRR).
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