Example sentences for: stratagene

How can you use “stratagene” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A primer complementary to the19 bp Tn 5 ME (5'-CTGTCTCTTATACACATCT-3') was used to amplify the transposons (1 cycle at 95°C for 3:30 min., 24 cycles of 95°C for 30 sec 47°C for 30 sec 72°C for 1 min., 1 cycle at 72°C for 5 min.) with Pfu polymerase (Stratagene, La Jolla, CA).

  • Top 10 F' E. coli (Stratagene) were transformed with 1 μL of the transposition reaction and plated on LB agar with either ampicillin (100 μg/mL) and kanamycin (50 μg/mL) to recover transposed clones, or ampicillin (100 μg/mL) alone to establish the transposition efficiency.

  • Probes were 32P-labeled using the Prime-It II kit (Stratagene).

  • Total RNA was extracted from frozen breast tissues and laser-captured materials using the StrataPrep Total RNA kit (Stratagene, La Jolla, CA, USA).

  • The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.


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