Example sentences for: stratagene

How can you use “stratagene” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA isolated from treated and untreated cell lines, and the total RNA and poly A +RNA from normal cervix obtained from Ambion (Austin, TX) was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).

  • Substitution of human myocilin Asn 57 with Ser was accomplished using the QuickChange XL Site-Directed Mutagenesis kit (Stratagene) according to the manufacturer's instructions.

  • Consecutive fractions of 0.5 ml each were collected after the void volume (7 ml), concentrated with StrataClean resin (Stratagene), and subjected to 10% SDS-PAGE followed by immunoblot analysis.

  • Prehybridization and hybridization were performed at 42°C and using Quick Hyb ®(Stratagene) as hybridization solution.

  • For first strand cDNA synthesis, 3 μg (unless otherwise specified) tumor total RNA, Universal Human Reference total RNA (Stratagene ®), or 150 ng poly(A) +RNA was mixed with 1 μg Eberwine primer (Operon ®) in RNase-free water to a total volume of 9 μl.


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