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Example sentences for: stratagene

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All PCR experiments were performed in a RoboCycler 96 Gradient with Hot Top Assembly (Stratagene, La Jolla, CA).
Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.
The insert size for this library is as expected using the Stratagene Hybrid-Zap kit, given that the size-selection column retains DNA molecules larger than 200 bp) (Stratagene technical support, personal communication).
The library was constructed using a Stratagene HybriZAP™ Library kit.
PCR amplification was performed in a 50 ul volume with the PfuTurbo Hotstart DNA Polymerase system (Stratagene) and included 0.2 mM dNTPs, 100 ng each primer, 2.5 units PfuTurbo DNA polymerase, 100 ng genomic DNA, and 1X cloned Pfu DNA polymerase reaction buffer.