Words similar to stratagene

Example sentences for: stratagene

How can you use “stratagene” in a sentence? Here are some example sentences to help you improve your vocabulary:

Prehybridization and hybridization were performed at 42°C and using Quick Hyb ®(Stratagene) as hybridization solution.
Poly(A) +RNA was prepared from 19 human carcinoma cell lines (2 brain, 5 colon, 10 ovarian, 1 pancreatic, and 1 testicular) and Stratagene's universal human reference RNA.
These cDNAs were gel-purified and used to produce 32P-labeled cDNA probes (Prime-It kit, Stratagene).
Total RNA isolated from the cell lines was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).
Oligonucleotide primers for the PCR reaction were based on the published sequence of murine Rab24 [ 24 ] . Kpn1 and BamH1 restriction sites were added to the 5' and 3' primers respectively, to facilitate cloning of the PCR product in frame with a 5' sequence cassette encoding a myc epitope (EQKLISEEDL), previously introduced into the pCMV5 expression vector [ 78 ] . The Rab24 cDNA was altered by overlap-extension PCR [ 79 ] , using appropriate mutator oligonucleotides and Pfu DNA polymerase (Stratagene Inc., La Jolla, CA), so as to introduce amino acid substitutions at D123 (D123I), T120 (T120A), S22 (S22N), S67 (S67Q), and a deletion of residues 127 through 130 (ΔEDRR).