Words similar to strand

Example sentences for: strand

How can you use “strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

Second strand cDNA synthesis was performed by mixing the first strand synthesis reaction with 106 μl RNase-free water, 15 μl 10X Advantage™ PCR buffer (Clontech), 3 μl 10 mM dNTP mix, 3 μl Advantage™ cDNA polymerase mix (Clontech), and 1 μl RNase H (2 U/μl, Invitrogen™).
First strand cDNA was then synthesized with the addition of 2μl 10 × RT buffer, 4μl dNTP mix, 1 Unit RNase inhibitor, and 100 Units MMLV-RT in a total volume of 20μl.
Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
The interpretation of this intra-strand rule is statistical rather than mechanical: if there are no biases in mutation and selection between the two strands, or if genes are distributed evenly between the two strands, a C→G mutation on one strand (for example) cannot be distinguished from a G→C mutation on the other.
Initially, the linear range of amplification for cyclooxygenase-2 (COX-2) and tumor necrosis factor alpha (TNF-α) mRNA was determined using mouse COX-2 and TNF-α primers (Ambion Inc., Austin, TX, USA) with the neutrophil first strand cDNA samples as templates, following the manufacturer's instructions.