Example sentences for: stopped-flow

How can you use “stopped-flow” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Stopped-flow fluorescence studies at 10°C with the N233L and N233D mutants and nitrocefin as substrate resulted in K S values of 26 ± 9 and 25 ± 8 μM, respectively.

  • Stopped-flow fluorescence studies at 10°C resulted in a K S value of 31 ± 11 μM for the I164A mutant.

  • Stopped-flow fluorescence studies at 10°C of nitrocefin hydrolysis by Y191A and Y191F resulted in K S values of 85 ± 9 and 22 ± 6 μM, respectively.

  • To probe further the binding of nitrocefin to wild-type L1, stopped-flow fluorescence studies were conducted as previously described [ 40 ] (Figure 3).

  • Recently, Spencer and co-workers reported that stopped-flow fluorescence studies can be used to monitor the reaction of L1 with nitrocefin and that an initial binding step can be directly monitored [ 40 ] . By increasing the concentration of nitrocefin, the rate of the initial binding step increased to a maximum, and fitting of these data yielded a binding constant (called K S herein) for nitrocefin.


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