Words similar to stopped-flow
Example sentences for: stopped-flow
How can you use “stopped-flow” in a sentence? Here are some example sentences to help you improve your vocabulary:
Each of the L1 mutants were studied using the stopped-flow fluorescence studies, and the resulting data were fitted as reported by Spencer et al.
Recently, Spencer and co-workers reported that stopped-flow fluorescence studies can be used to monitor the reaction of L1 with nitrocefin and that an initial binding step can be directly monitored [ 40 ] . By increasing the concentration of nitrocefin, the rate of the initial binding step increased to a maximum, and fitting of these data yielded a binding constant (called K S herein) for nitrocefin.
Absorbance data were converted to concentration data as described previously by McMannus and Crowder [ 39 ] . Stopped-flow fluorescence studies of nitrocefin hydrolysis by L1 were performed on an Applied Photophysics SX.
By using this mechanism [ 39 ] , K m is equal to {(k -1 + k 2 )k 3 k 4 } / {k 1 (k 3 k 4 + k 2 k 4 + k 2 k 3 }. To probe more directly the reaction, stopped-flow absorbance studies were conducted, and the substrate decay rates (390 nm) were studied as a function of nitrocefin concentration.
Stopped-flow fluorescence studies at 10°C resulted in a K S value of 31 ± 11 μM for the I164A mutant.
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