Words similar to stopped-flow
Example sentences for: stopped-flow
How can you use “stopped-flow” in a sentence? Here are some example sentences to help you improve your vocabulary:
Absorbance data were converted to concentration data as described previously by McMannus and Crowder [ 39 ] . Stopped-flow fluorescence studies of nitrocefin hydrolysis by L1 were performed on an Applied Photophysics SX.
Stopped-flow fluorescence studies at 10°C resulted in a K S value of 31 ± 11 μM for the I164A mutant.
also reported stopped-flow fluorescence studies when using cefaclor and meropenem as substrates, and K S values for these substrates were reported to be 710 ± 180 and 272 ± 112 μM, respectively [ 40 ] . However in our hands, the rates of substrate hydrolysis were so fast when using wild-type L1 that we could not use substrate concentrations high enough to saturate the enzyme.
By using this mechanism [ 39 ] , K m is equal to {(k -1 + k 2 )k 3 k 4 } / {k 1 (k 3 k 4 + k 2 k 4 + k 2 k 3 }. To probe more directly the reaction, stopped-flow absorbance studies were conducted, and the substrate decay rates (390 nm) were studied as a function of nitrocefin concentration.
Each of the L1 mutants were studied using the stopped-flow fluorescence studies, and the resulting data were fitted as reported by Spencer et al.
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