Words similar to stopped-flow

Example sentences for: stopped-flow

How can you use “stopped-flow” in a sentence? Here are some example sentences to help you improve your vocabulary:

Stopped-flow fluorescence studies at 10°C of nitrocefin hydrolysis by Y191A and Y191F resulted in K S values of 85 ± 9 and 22 ± 6 μM, respectively.
By using this mechanism [ 39 ] , K m is equal to {(k -1 + k 2 )k 3 k 4 } / {k 1 (k 3 k 4 + k 2 k 4 + k 2 k 3 }. To probe more directly the reaction, stopped-flow absorbance studies were conducted, and the substrate decay rates (390 nm) were studied as a function of nitrocefin concentration.
Stopped-flow fluorescence studies at 10°C resulted in a K S value of 31 ± 11 μM for the I164A mutant.
also reported stopped-flow fluorescence studies when using cefaclor and meropenem as substrates, and K S values for these substrates were reported to be 710 ± 180 and 272 ± 112 μM, respectively [ 40 ] . However in our hands, the rates of substrate hydrolysis were so fast when using wild-type L1 that we could not use substrate concentrations high enough to saturate the enzyme.
Recently, Spencer and co-workers reported that stopped-flow fluorescence studies can be used to monitor the reaction of L1 with nitrocefin and that an initial binding step can be directly monitored [ 40 ] . By increasing the concentration of nitrocefin, the rate of the initial binding step increased to a maximum, and fitting of these data yielded a binding constant (called K S herein) for nitrocefin.