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Example sentences for: stoffel

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The Stoffel fragment of Taq polymerase is a truncated portion of the enzyme which retains polymerase activity but lacks the 5'->3' exonuclease activity of Taq polymerase [ 14 ] . The Stoffel fragment does not cut at the lac operator as does Taq polymerase [ 3 ] . It might therefore be a good choice of polymerase for jumping PCR.
Consistent with the expectation, results of using the Stoffel fragment, without additives such as gp32, (NH 4 ) 2 SO 4 or detergent, gave a S/N of 100 (See figure 4, compare intact lanes 3, 4, 5, 6 to cut lanes 7,8,9,10).
The Stoffel fragment yielded a S/N of 1% without modifications of the standard protocol but was not further improved by buffer modifications or gp32.
The Stoffel fragment of Taq polymerase gave a S/N of approximately 100
The buffer for Taq polymerase including (NH 4 ) 2 SO 2 and for the Stoffel fragment had a low KCl concentration (10 mM).