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Example sentences for: stoffel

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However, the Stoffel fragment had low output in BPCR between primers b1 and M13 pI.
The source templates used were also prepared by standard PCR with the Stoffel fragment and the primer pair b1 and Jp on pBS (see figure 2).
The Stoffel fragment of Taq polymerase is a truncated portion of the enzyme which retains polymerase activity but lacks the 5'->3' exonuclease activity of Taq polymerase [ 14 ] . The Stoffel fragment does not cut at the lac operator as does Taq polymerase [ 3 ] . It might therefore be a good choice of polymerase for jumping PCR.
The buffer for Taq polymerase including (NH 4 ) 2 SO 2 and for the Stoffel fragment had a low KCl concentration (10 mM).
The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.