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Example sentences for: stoffel

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The Stoffel fragment of Taq polymerase is a truncated portion of the enzyme which retains polymerase activity but lacks the 5'->3' exonuclease activity of Taq polymerase [ 14 ] . The Stoffel fragment does not cut at the lac operator as does Taq polymerase [ 3 ] . It might therefore be a good choice of polymerase for jumping PCR.
The source templates used were also prepared by standard PCR with the Stoffel fragment and the primer pair b1 and Jp on pBS (see figure 2).
The Stoffel fragment yielded a S/N of 1% without modifications of the standard protocol but was not further improved by buffer modifications or gp32.
In this size range the Stoffel fragment of Taq polymerase yielded a better S/N than Taq polymerase.
Consistent with the expectation, results of using the Stoffel fragment, without additives such as gp32, (NH 4 ) 2 SO 4 or detergent, gave a S/N of 100 (See figure 4, compare intact lanes 3, 4, 5, 6 to cut lanes 7,8,9,10).