Example sentences for: step-in

How can you use “step-in” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Figure 5presents LM-PCR displays in which both the left-arm- and right-arm-distal TAS regions show single bands, "step-in" verified.

  • For further specificity, a second PCR reaction is sometimes run on the P1-7A product, using a "step-in" or nested primer, "P2."

  • Furthermore, these clone 3.5 patterns are displayed in two parallel lanes which illustrate the band-confirmation technique of "telomere step-in PCR."

  • "Step-in" verification PCR is performed on 0.1 μl of P1+7A product, again with P1 but Tel instead of 7A.

  • an appropriate nested primer is 5'- 32P-labeled as previously described [ 54 ] ; 5 μl of the P1+7A reaction or the step-in verification reaction is denatured and annealed with 1 pmol of the end-labeled display primer and 0.5 units of Taq polymerase (Qiagen) in 10 μl final volume of 1× "Taq salts" (Qiagen).

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