Words similar to stacking
Example sentences for: stacking
How can you use “stacking” in a sentence? Here are some example sentences to help you improve your vocabulary:
The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.
Only a very few sewing operations involve a machine that is fully automated, in which the operator’s job comes down to stacking parts at one end of the sewing system and re-threading the machines if a thread breaks.
Lane et al . reported recently that the Golgi complex in HeLa cells is fragmented into scattered vesicles by caspases due to interference of Golgi reassembly and abnormalities of the 65 kDa stacking protein GRASP65 [ 32 ] . In the present report, we describe characteristic changes of the Golgi complex during the progression of apoptosis.
This is probably the consequence of various sequence-dependent effects: first, the difference in stacking energies of single-stranded snippets between the PM and the MM sequences can easily be in the range of the gain in binding energies; second, there are certainly kinetic effects as the hybridizations are not carried to complete equilibrium; and third, there is always the possibility of sequence-dependent synthesis efficiencies.
RAW cells expressing IDO and vector only controls were harvested in cell lysis buffer (PBS, 1%NP40, 0.5% sodium deoxycholate, 0.1% SDS, 150 ng/ml PMSF, 100 ng/ml aprotinin) and 25 μg of cell protein was electrophoresed on 10% polyacrylamide gels overlayed with a 5% stacking gel.
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