Example sentences for: stacking

How can you use “stacking” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • By rhetorically stacking their side of the question (to include husbands, doctors, and clergymen, along with women) and defining the other side as "the government," pro-choicers have won the sympathy of voters who dislike big government more than they dislike abortion.

  • Allele specificity of circularization is dependent upon two factors: 1) favorable hybridization of the matching OCP because of the correct base pair formed at the 3' end and also because of base stacking between the 3' and 5' annealed ends, and 2) specificity of the ligase for correctly base paired ends.

  • Oligodeoxyribonucleotide probes have been found to form duplexes with non-helical properties on positively charged surfaces [ 14 ] . These duplexes are highly asymmetrical and unwound, possibly incurring a significant loss in base stacking which may subsequently affect the energetics of duplex formation.

  • The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.

  • Cross-validation tests of 14 different properties such as DNA bendability and nucleosome positioning (compiled in [ 46]) showed that stacking energy delivered the best classification results for both human and Drosophila sequences when combined with the sequence probabilities.


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