Example sentences for: stacking

How can you use “stacking” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Allele specificity of circularization is dependent upon two factors: 1) favorable hybridization of the matching OCP because of the correct base pair formed at the 3' end and also because of base stacking between the 3' and 5' annealed ends, and 2) specificity of the ligase for correctly base paired ends.

  • Only a very few sewing operations involve a machine that is fully automated, in which the operator’s job comes down to stacking parts at one end of the sewing system and re-threading the machines if a thread breaks.

  • RAW cells expressing IDO and vector only controls were harvested in cell lysis buffer (PBS, 1%NP40, 0.5% sodium deoxycholate, 0.1% SDS, 150 ng/ml PMSF, 100 ng/ml aprotinin) and 25 μg of cell protein was electrophoresed on 10% polyacrylamide gels overlayed with a 5% stacking gel.

  • (These conspiracies are stacking up like Russian nesting dolls: Right-wingers advance conspiracy theories about Whitewater; the administration shoots back with a conspiracy theory about the right-wingers' conspiracy theories; now the Journal counters with a conspiracy theory about the administration's conspiracy theory about right-wingers' conspiracy theories.

  • 10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.


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