Words similar to stacking
Example sentences for: stacking
How can you use “stacking” in a sentence? Here are some example sentences to help you improve your vocabulary:
Ongoing research into this problem interprets hybridization data not only in terms of Watson-Crick base pairing, but also in terms of base stacking interactions, loops, bridges, and dangling ends - and in the case of DNA immobilized on nylon membranes - in terms of diffusion of solvents into and out of membrane pores, multiple interactions within pores, and details of the way in which the DNA is attached to its membrane support [ 8 ] .
The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.
As for the square brass monkey with circular depressions for stacking cannonballs, it would have to contract a great deal more than the iron balls to cause them to tumble.
RAW cells expressing IDO and vector only controls were harvested in cell lysis buffer (PBS, 1%NP40, 0.5% sodium deoxycholate, 0.1% SDS, 150 ng/ml PMSF, 100 ng/ml aprotinin) and 25 μg of cell protein was electrophoresed on 10% polyacrylamide gels overlayed with a 5% stacking gel.
The close proximity of Lys61 to both A-4 and A-10 suggests the possibility that the introduction of aromatic residues may allow stacking interactions.
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