Words similar to sprague-
Example sentences for: sprague-
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Since each individual sample was pooled from 5 male Sprague-Dawley animals of a similar age and from a single supplier (Charles River), biological variation is likely to be minimal.
Adult female Sprague-Dawley rats (2-3 months of age) were housed (four animals per cage) in isolation under controlled lighting conditions (14 h of light, 10 hr of darkness; lights on from 0500 to 1900 h) at 23°C and provided formulated rat chow and water ad libitum . Vaginal smears were taken daily, and rats exhibiting two or more consecutive 4-day estrous cycles were used.
Eleven 3-month-old Sprague-Dawley rats underwent emphysema induction by a single intratracheal instillation of porcine pancreatic elastase (ICN Biochemicals, Cleveland, Ohio) at 25 units per 100 g body weight, diluted in 0.60 ml of normal saline as described previously [ 4 ] . Two animals died on the night after instillation, with evidence at autopsy the following day of diffuse pulmonary hemorrhage.
Primary rat aortic smooth muscle cells (RASMCs) were isolated from 12-16 week old Sprague-Dawley rats as described previously [ 51 ] . RASMCs were cultured in DMEM supplemented with 10% FBS and glutamine Pen-Strep.
Sympathetic neuronal cultures were obtained by dissociating superior cervical ganglia from perinatal (21 day embryos to 1 day postnatal) Holtzman rats (Harlan Sprague-Dawley, Indianapolis), using previously described methods [ 13].