Example sentences for: sporulated

How can you use “sporulated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The Leu +diploid transformants were selected and then sporulated.

  • The diploids were sporulated, and if ~50% of the MEN-deleted spores were resistant to 5-FOA, then net1-1 could bypass the MEN null mutation.

  • We then sporulated the heterozygotes, dissected tetrads, and scored for spore viability and the presence of the kanMX marker.

  • Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [ 19 ] . The plasmids were transformed into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pct1::kanMX diploids using the lithium acetate method [ 18 ] . The Leu +diploid transformants were then sporulated on ME plates at room temperature.

  • Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.


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