Example sentences for: sporulated

How can you use “sporulated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resultant MDN1(HA)/MDN1 diploid strain was sporulated and the tetrads dissected.

  • To assign tab mutants to linkage groups, cdc15Δ tab [pMET3-CDC15, URA3 ] strains were crossed against each other, and the diploids were sporulated and dissected.

  • Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [ 19 ] . The plasmids were transformed into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pct1::kanMX diploids using the lithium acetate method [ 18 ] . The Leu +diploid transformants were then sporulated on ME plates at room temperature.

  • We then sporulated the heterozygotes, dissected tetrads, and scored for spore viability and the presence of the kanMX marker.

  • The diploids were sporulated, and if ~50% of the MEN-deleted spores were resistant to 5-FOA, then net1-1 could bypass the MEN null mutation.


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