Example sentences for: sporulated

How can you use “sporulated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We then sporulated the heterozygotes, dissected tetrads, and scored for spore viability and the presence of the kanMX marker.

  • To assign tab mutants to linkage groups, cdc15Δ tab [pMET3-CDC15, URA3 ] strains were crossed against each other, and the diploids were sporulated and dissected.

  • Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [ 19 ] . The plasmids were transformed into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pct1::kanMX diploids using the lithium acetate method [ 18 ] . The Leu +diploid transformants were then sporulated on ME plates at room temperature.

  • The resultant MDN1(HA)/MDN1 diploid strain was sporulated and the tetrads dissected.

  • Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.


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