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Example sentences for: spo
How can you use “spo” in a sentence? Here are some example sentences to help you improve your vocabulary:
It is dispensable for chromosome pairing and synapsis in some organisms, but is required in others [ 22 24 25 26 27 28 ] . Budding yeast spo11Δ (null) mutants lack chromosome pairing, but catalytic site tyrosine mutants ( spo11-Y135F ) support significant levels of DSB-independent pairing [ 29 ] , so the function of Spo11 proteins in pairing may be separable from its function in catalyzing recombination.
Meiotic progression in spo11 ( rec12 ) null mutants of budding yeast is accelerated [ 29 ] , supporting our hypothesis that Rec12 may participate in an "MI nondisjunction checkpoint."
Presence of four introns suggested that the rec12 +cDNA encodes a protein of 345 amino acids with high homology to eukaryotic Spo11 proteins (Figure 1B).
Rec12 (Spo11) protein shares homology with the catalytic (Top6A) subunit of Topoisomerase VI, which functions as a heterotetramer with Top6B [ 16 17 ] . However, no Top6B homolog is present in most eukaryotes and the Rec12 (Spo11) enzyme does not need to affect strand passage in the manner of canonical type II topoisomerases [ 11 ] . We therefore tested whether overexpression of rec12 +would lead to a hyper-recombination phenotype, thereby implicating function as a homodimer.
Thus, with regard to primary protein sequence (Figure 1), inferred subunit structure (Figure 2and data not shown), strict requirement for meiotic recombination (Figure 2, Table 1), and dependence upon its active site tyrosine (Table 1), Rec12 protein is a structurally and functionally conserved member of the Spo11 family of enzymes that catalyze meiotic dsDNA cleavage to initiate recombination [ 11 ] .
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