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In cases where Probit Analysis is not appropriate, the LC50 and associated confidence interval may be estimated by the Spearman-Karber Method (Appendix J) or the Trimmed Spearman-Karber Method (Appendix K).
The recommended statistical analysis of most data from chronic toxicity tests with aquatic organisms follows a decision process illustrated in the flowchart in Figure 2. An initial decision is made to use point estimation techniques (the Probit Analysis, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, the Graphical Method, or Linear Interpolation Method) and/or to use hypothesis testing (Dunnett's Test, the t test with the Bonferroni adjustment, Steel's Many-one Rank Test, or Wilcoxon Rank Sum Test with the Bonferroni adjustment).
4 LC50 estimated by Probit, Litchfield-Wilcoxon, or Trimmed Spearman-Karber method.
NOECs and LOECs are determined by hypothesis testing (Dunnett's Test, a t test with the Bonferroni adjustment, Steel's Many-one Rank Test, or the Wilcoxon Rank Sum Test with the Bonferroni adjustment), whereas LCs, ICs, and ECs are determined by point estimation techniques (Probit Analysis, Spearman-Karber Method, Trimmed Spearman-Karber Method, Graphical Method or Linear Interpolation Method).
In cases where Probit Analysis is not appropriate, the LC50 and confidence interval may be estimated by the Spearman-Karber Method (Appendix I) or the Trimmed Spearman-Karber Method (Appendix J).