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NOECs and LOECs are determined by hypothesis testing (Dunnett's Test, a t test with the Bonferroni adjustment, Steel's Many-one Rank Test, or the Wilcoxon Rank Sum Test with the Bonferroni adjustment), whereas LCs, ICs, and ECs are determined by point estimation techniques (Probit Analysis, Spearman-Karber Method, Trimmed Spearman-Karber Method, Graphical Method or Linear Interpolation Method).
, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, or the Graphical Method) (see Appendices I-L and the discussion below).
LC50s were estimated by the graphical or Spearman-Karber method.
As a compromise between the practicality of high doses and the sensitivity associated with realistically low doses, we propose the ID 50 . The ID 50 can be estimated using well-established nonparametric methods like Spearman-Kärber [40] or single-parameter methods [41], and there is software available, such as a freely distributed package called ID50 developed by John Spouge (http://www.ncbi.nlm.nih.gov/CBBresearch/Spouge/Virology/, which allows an automated estimation of the ID 50 from data generated in titration experiments.
In cases where Probit Analysis is not appropriate, the LC50 and confidence interval may be estimated by the Spearman-Karber Method (Appendix I) or the Trimmed Spearman-Karber Method (Appendix J).