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In cases where Probit Analysis is not appropriate, the LC50 and associated confidence interval may be estimated by the Spearman-Karber Method (Appendix J) or the Trimmed Spearman-Karber Method (Appendix K).
, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, or the Graphical Method) (see Appendices I-L and the discussion below).
The recommended statistical analysis of most data from chronic toxicity tests with aquatic organisms follows a decision process illustrated in the flowchart in Figure 2. An initial decision is made to use point estimation techniques (the Probit Analysis, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, the Graphical Method, or Linear Interpolation Method) and/or to use hypothesis testing (Dunnett's Test, the t test with the Bonferroni adjustment, Steel's Many-one Rank Test, or the Wilcoxon Rank Sum Test with the Bonferroni adjustment).
As a compromise between the practicality of high doses and the sensitivity associated with realistically low doses, we propose the ID 50 . The ID 50 can be estimated using well-established nonparametric methods like Spearman-Kärber [40] or single-parameter methods [41], and there is software available, such as a freely distributed package called ID50 developed by John Spouge (http://www.ncbi.nlm.nih.gov/CBBresearch/Spouge/Virology/, which allows an automated estimation of the ID 50 from data generated in titration experiments.
The recommended statistical analysis of most data from chronic toxicity tests with aquatic organisms follows a decision process illustrated in the flowchart in Figure 2. An initial decision is made to use point estimation techniques (the Probit Analysis, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, the Graphical Method, or Linear Interpolation Method) and/or to use hypothesis testing (Dunnett's Test, the t test with the Bonferroni adjustment, Steel's Many-one Rank Test, or Wilcoxon Rank Sum Test with the Bonferroni adjustment).
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