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, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, or the Graphical Method) (see Appendices I-L and the discussion below).
The recommended statistical analysis of most data from chronic toxicity tests with aquatic organisms follows a decision process illustrated in the flowchart in Figure 2. An initial decision is made to use point estimation techniques (the Probit Analysis, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, the Graphical Method, or Linear Interpolation Method) and/or to use hypothesis testing (Dunnett's Test, the t test with the Bonferroni adjustment, Steel's Many-one Rank Test, or Wilcoxon Rank Sum Test with the Bonferroni adjustment).
In cases where Probit Analysis is not appropriate, the LC50 and associated confidence interval may be estimated by the Spearman-Karber Method (Appendix J) or the Trimmed Spearman-Karber Method (Appendix K).
, the Spearman-Karber Method, the Trimmed Spearman-Karber Method, or the Graphical Method) (see Appendices H-K) (see discussion below).
In cases where Probit Analysis is not appropriate, the LC50 and confidence interval may be estimated by the Spearman-Karber Method (Appendix I) or the Trimmed Spearman-Karber Method (Appendix J).