Words similar to spanned
Example sentences for: spanned
How can you use “spanned” in a sentence? Here are some example sentences to help you improve your vocabulary:
cDNA prepared from 0.5 μg RNA was subjected to PCR using murine gene-specific primers for 8 different cytokines and chemokines (Table 1) as previously detailed [ 18 ] . All of the primer pairs spanned at least one intron in the corresponding genomic DNA.
With an estimated gap size, LBNL used one of five gap-filling methods to determine their sequence: first, gaps less than 500 bp were sequenced using custom primers designed by consed and a 3-kb or 10-kb plasmid as DNA template; second, gaps between 500 bp and 2.5 kb that were spanned by a 3-kb clone were completed by sequencing the 3-kb clone using a transposon-based sequencing strategy, described below; third, gaps between 2.5 kb and 10 kb that were spanned by a 10-kb clone were completed by sequencing the 10-kb clone using a transposon-mediated sequencing strategy; fourth, gaps larger than 10 kb were completed by sequentially sequencing multiple 10-kb clones to walk across the gap.
The remaining datasets were considered in the following order and resulted in an additional one or two points: the cDNA and annotation data were analyzed to determine if any entry in this class spanned the entire length of the CDS; if so, an additional two points were assigned, and if not, GenBank/EMBL/DDBJ and error report entries were analyzed and if any spanned the length of CDS, two points were assigned; if none of these data classes corresponded to the full-length CDS, then the existence of partial cDNA data and/or overlapping EST data merited one point.
Our assay investigated tissues that spanned most of this period (see Methods), including four time points in the critical period surrounding P10 to P18 (P10, P12, P14 and P18).
To further investigate this possibility, we performed RT-PCR to generate cDNAs that spanned exon 20 of EGFR and included sequences from exon 19 and 21.
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