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Example sentences for: spacers
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Also, a total of 20 protein-binding sites were annotated within E. coli spacers orthologous to Buchnera spacers (that is, with the same flanking genes in the same orientation).
Considering only spacers within syntenic fragments, spacer length does not increase further when the number of genes lost is greater than one (Figure 8).
The sequence boundaries of the spacers were determined by comparison to published Artemisia sequences [ 34 ] , and complete sequences were deposited in GenBank (see additional file 1).
[ 76 ] , as previously described [ 34 ] . For unpublished sequences, the spacers were sequenced on an ABI PRISM 310 ®Genetic Analyzer using capillary sequencing and Big Dye Terminator Chemistry (Applied Biosystems, Inc., Foster City, CA).
In 44 of the orthologous spacers, a σ-70 promoter is annotated in E. coli on the basis of experimental evidence and/or presence of a promoter consensus sequence [ 14].