Example sentences for: sonication

How can you use “sonication” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • ChIP DNA was fragmented by sonication and isolated using antibody against di-methyl-H3 K4 as described previously [ 6 ] . Restricted genomic DNA was prepared as follows: yeast genomic DNA isolated by bead lysis, phenol/chloroform extraction, and ethanol precipitation, was restricted either with Alu I or with Rsa I (New England BioLabs (NEB)).

  • Cells were resuspended in lysis buffer (10 mM Tris (pH 7.5), 1 mM EDTA, 50 mM NaCl, 0.25% Nonidet P-40, 1 mM PMSF, 1 mg of aprotinin/mL, 0.1 mM sodium vanadate, 10 mM sodium molybdate, and 10 mM β-glycerol phosphate) and lysed by sonication with a microtip on setting 2 and 20% duty cycle for 10 s.

  • Sonication was carried out on ice using an ultrasonicator processor (PGC Scientific, Caithersburg, MA, USA), and the mixtures were examined microscopically for complete break of the nuclei.

  • One part was resuspended in 5 ml of 50 mM potassium phosphate buffer (pH 7.0), and broken by sonication to make crude extract.

  • Synovial tissue homogenization was completed on ice using a motorized homogenizer, followed by sonication for 30 seconds.


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