Words similar to sonication
Example sentences for: sonication
How can you use “sonication” in a sentence? Here are some example sentences to help you improve your vocabulary:
ChIP DNA was fragmented by sonication and isolated using antibody against di-methyl-H3 K4 as described previously [ 6 ] . Restricted genomic DNA was prepared as follows: yeast genomic DNA isolated by bead lysis, phenol/chloroform extraction, and ethanol precipitation, was restricted either with Alu I or with Rsa I (New England BioLabs (NEB)).
Cells were resuspended in lysis buffer (10 mM Tris (pH 7.5), 1 mM EDTA, 50 mM NaCl, 0.25% Nonidet P-40, 1 mM PMSF, 1 mg of aprotinin/mL, 0.1 mM sodium vanadate, 10 mM sodium molybdate, and 10 mM β-glycerol phosphate) and lysed by sonication with a microtip on setting 2 and 20% duty cycle for 10 s.
Sonication was carried out on ice using an ultrasonicator processor (PGC Scientific, Caithersburg, MA, USA), and the mixtures were examined microscopically for complete break of the nuclei.
One part was resuspended in 5 ml of 50 mM potassium phosphate buffer (pH 7.0), and broken by sonication to make crude extract.
Synovial tissue homogenization was completed on ice using a motorized homogenizer, followed by sonication for 30 seconds.
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