Words similar to sonication
Example sentences for: sonication
How can you use “sonication” in a sentence? Here are some example sentences to help you improve your vocabulary:
The frozen cell pellets from 500 ml spinner flask culture were lysed by sonication in Buffer A (50 mM Tris-HCL pH 8, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 μg/ml leupeptin, pepstatin, and E64, and 1 mM PMSF) containing 450 mM NaCL and 1% NP40.
Bath-type sonication was then used to generate suspensions of vesicles of these lipids in PBS.
Sonication was carried out on ice using an ultrasonicator processor (PGC Scientific, Caithersburg, MA, USA), and the mixtures were examined microscopically for complete break of the nuclei.
Briefly, cells were suspended in 50 mM Tris, pH 8.0, 100 mM NaCl, 1 mM EDTA, 1 mM PMSF, treated with 0.4 mg/mL lysozyme and lysed by freeze-thaw and sonication.
The cells were disrupted by sonication using an Ultratip Labsonic System (Lab-Line Instruments), and then centrifuged at 150,000 × g for 65 min at 4°C.