Words similar to smegmatis
Example sentences for: smegmatis
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Because the cosmids integrate into the mycobacteriophage L5 attachment site in the M. smegmatis genome, a single copy of the M. tuberculosis DNA is maintained in the M. smegmatis transformants [ 20 ] .
[ 31 ] compared the expression of the luciferase gene in M. paratuberculosis with the fast-growing M. smegmatis and concluded that the rates of transcription and translation may not account for the slow growth of M. paratuberculosis .
Construction of σ E-over-expressing strain of M. smegmatis
The relaxation and decatenation activities of T. maritima topoisomerase I appear to be significantly more efficient than those of the E. coli topoisomerase I [ 33 ] . Based on their primary sequences, a number of bacterial topoisomerase I enzymes do not appear to coordinate any Zn(II) with tetracysteines motifs while other type IA topoisomerase has up to 4 tetracysteine motifs [ 7 ] . The topoisomerase I from Mycobacterium smegmatis has been demonstrated biochemically not to bind Zn(II) [ 34 ] . In contrast, mutation disrupting the fourth Zn(II) motif of Helicobacter pylori topoisomerase I abolished enzyme function in vivo [ 35 ] . Therefore there may be significant differences in the mechanisms of type IA topoisomerases from different organisms with respect to requirement of Zn(II) binding for relaxation activity.
The M. smegmatis coding sequence, gnd , has similarity to the 6-phophogluconate dehydrogenase genes in E. coli , but the mycobacterial protein is predicted to be about 200 amino acids shorter than the E. coli homolog . The length of non-coding intergenic regions between rpmH - dnaA and dnaA - dnaN is well conserved among the bacteria shown in figure 2. In many bacteria where a functional oriC has been identified, this gene order is conserved and oriC is adjacent to the dnaA gene [ 9 10 19 ] .
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