Words similar to smegmatis
Example sentences for: smegmatis
How can you use “smegmatis” in a sentence? Here are some example sentences to help you improve your vocabulary:
The M. smegmatis strain LR222 was obtained from Dr. Jack Crawford, Tuberculosis and Mycobacteriology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA.
However, repeated attempts to electroporate the 4 kb plasmid into M. smegmatis LR222 did not generate any stable kanamycin-resistant transformants (data not shown).
4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.
To enrich for clones with increased survival in human macrophages, the library was passed through THP-1 macrophages as shown schematically in Figure 1. THP-1 is a human monocyte-derived macrophage cell line [ 21 ] and wild-type M. smegmatis bacteria are rapidly killed by THP-1 macrophages [ 22 ] . After three rounds of enrichment, the resulting clones were analyzed individually by Southern blot to evaluate the number of different clones present as previously described [ 20 ] . Out of the 3000 colonies recovered, genomic DNAs from the bacteria of 90 randomly chosen colonies were digested with Pst I and hybridized with the pYUB178 vector.
BLAST searches of finished and unfinished microbial genomes http://www.ncbi.nlm.nih.gov/Microb_blast/yielded additional sequences in the genomes of Corynebacterium diphtheriae , Mycobacterium avium , M. bovis , and M. smegmatis with significant NA homology to Ps-moeZ , but these were not included in this study because of their potentially provisional nature, pending completion of the genome sequencing projects.
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