Words similar to smegmatis
Example sentences for: smegmatis
How can you use “smegmatis” in a sentence? Here are some example sentences to help you improve your vocabulary:
The relaxation and decatenation activities of T. maritima topoisomerase I appear to be significantly more efficient than those of the E. coli topoisomerase I [ 33 ] . Based on their primary sequences, a number of bacterial topoisomerase I enzymes do not appear to coordinate any Zn(II) with tetracysteines motifs while other type IA topoisomerase has up to 4 tetracysteine motifs [ 7 ] . The topoisomerase I from Mycobacterium smegmatis has been demonstrated biochemically not to bind Zn(II) [ 34 ] . In contrast, mutation disrupting the fourth Zn(II) motif of Helicobacter pylori topoisomerase I abolished enzyme function in vivo [ 35 ] . Therefore there may be significant differences in the mechanisms of type IA topoisomerases from different organisms with respect to requirement of Zn(II) binding for relaxation activity.
Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).
4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene and the lacZ gene.
To develop the I-TRAP method for identifying M. tuberculosis promoters dependent on the expression of σ E, a strain of M. smegmatis over-expressing the M. tuberculosis σ E, a σ Emutant strain of M. smegmatis, and a M. tuberculosis promoter-trap library were created.
Using an enrichment and screening procedure, two M. tuberculosis genes, Rv0365c and Rv2235, were identified that could confer an enhanced ability to survive in human macrophages to normally susceptible M. smegmatis recipients.
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