Words similar to smai

Example sentences for: smai

How can you use “smai” in a sentence? Here are some example sentences to help you improve your vocabulary:

After polishing the ends of the fragments using Klenow (New England Biolabs, Beverly, MA), they were cloned into SmaI -restricted/CIAP pUC18 vector.
pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
pEGFP-N346 was generated by inserting a HindIII-NdeI fragment (containing amino acid 70-416 of MmCOP1; NdeI site was filled in by Klenow DNA polymerase) into pEGFP vector digested by HindIII and SmaI.
This assay employed transfection of a plasmid containing the 230 bp SmaI fragment of ori s , the HSV origin of replication, and a LacZ reporter gene, into Vero cells.
The resulting fragment (hCARΔcyt) was restriction endonuclease digested and subcloned into the EcoRI/SmaI site of the VA human CD2 mini-gene containing the human CD2 promoter and 3' LCR [ 16 ] . Automated sequencing (Applied Biosystems, Foster City, CA; University of Alabama at Birmingham Department of Microbiology sequencing facility) of the entire construct confirmed its accuracy.