Words similar to skp
Example sentences for: skp
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Consistent with this idea, biochemical studies based on the crystal structure of the human SKP1-SKP2 complex revealed cooperation of the SKP2 F-box with an adjacent region in binding of SKP1 [ 28 ] . Consistent with this finding, certain truncation mutants of the budding yeast F-box proteins Grr1p and Cdc4p interact poorly with Skp1p, despite the retainment of their F-boxes [ 30 31 ] . On the other hand, the F-box of Pop1p is essential for Rum1p degradation (Fig.
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
CUL1, in turn binds to SKP1, and together with HRT1, mediates the interaction with the ubiquitin-conjugating enzyme CDC34/UBC3.
Although, in three independent experiments, the fraction of cells in S phase was significantly (p = 0.009) higher in MIB-arrested cells overexpressing SKP2 (7.
Complementary DNA fragments for SKP2, p27, and GAPDH were amplified by PCR and radiolabeled by random priming.
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