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Example sentences for: sk-n-be
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SK-N-BE neuroblastoma cells, Jurkat (TIB-152) and Jiyoye (CCL-87) cells were obtained from the ATCC (Manassas, VA).
In addition, the same three peptides were found to be cytotoxic to SK-N-BE cells in the absence of TNFα (Figure 4a).
It has been shown that TNFα induced proliferation of the undifferentiated neuroblastoma cell line, SK-N-BE, resulted from activation of TNFR2 [ 30 ] . Proliferation of SK-N-BE cells could be suppressed by the pre-incubation of cells with anti-TNFR2 blocking antibodies without concomitant cytotoxicity (data not shown).
SK-N-BE and Jurkat cells were maintained in RPMI 1640 media supplemented with 10% fetal bovine serum (FBS) and 2 mM GlutaMAX™-I, while Jiyoye cells were maintained in RPMI 1640 media supplemented with 15% FBS and 2 mM GlutaMAX™.
SK-N-BE cells were seeded loosely in 96 well plates in SK-N-BE medium and allowed to grow to 75-80% confluency.