Words similar to single-pass
Example sentences for: single-pass
How can you use “single-pass” in a sentence? Here are some example sentences to help you improve your vocabulary:
, all sequences were single-pass and exactly as the sequencer software, ABI Prism Sequencing Analysis v. 3.3, called them).
Sequencing of Expressed Sequence Tags (ESTs) has resulted in the rapid identification of expressed genes [ 1 ] . ESTs are single-pass, partial sequences of cDNA clones from a large number of disease and normal tissue libraries.
The generation of expressed sequence tags (ESTs) by single-pass random sequencing of cDNA libraries is a powerful tool for rapid gene transcript identification in metazoans [ 14 15 16 17 ] including parasitic nematodes of humans and animals [ 18 19 20 21 22 23 ] . High-throughput projects on two dozen nematode species have now brought the total number of publicly available roundworm ESTs to nearly 400,000, with half the sequences coming from parasites [ 24 25 26 27 ] . As a part of these efforts, EST sequencing from plant parasitic nematodes is in progress [ 28 ] and pilot EST datasets from the root-knot nematode M. incognita and the cyst nematodes Globodera rostochiensis and G. pallida second-stage larvae have recently been analyzed [ 29 30 ] .
Thousands of EST libraries, consisting in sum of millions of single-pass sequence reads, have been generated by investigators from around the world, using a variety of methods, and deposited into public sequence databases.
It eventually became clear that, as the P. infestans sequences were all single-pass reads from the 5' end of a clone generated with the T3 primer, few sequences complementary to the 3' end of the mRNA sequence were present in the training set.