Words similar to single-copy
Example sentences for: single-copy
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7 times coverage from RPCI-43 and CHORI-251 segment 1 BAC library filters) were performed with a probe derived from CHRNA7 -related sequence, yielding a total of four positives, which was consistent with a single-copy locus (data not shown).
The problem of random transgene integration can be overcome by targeting transgenes to a specific genomic locus by homologous recombination in ES cells [ 2 3 4 ] . A method that allows the introduction of single copy transgenes into the X-linked hprt locus has been described previously by Bronson et al [ 2 ] . In this approach single-copy transgenes are introduced 5' of the hprt locus by homologous recombination in ES cells.
Since the basic single-copy repertoire of dispersed aromatic-pathway genes shown in Figure 2for Synechocystis (Ssp) is representative of other cyanobacteria such as Synechococcus (Syn) and Prochlorococcus (Pmu) and is also present at dispersed extra-operonic loci of Anabaena and Nostoc , an obvious possibility would seem to be that the genes of the supraoperon originated by LGT in a common ancestor of Anabaena and Nostoc . If so, speciation was followed by different species-specific gene-insertion events.
The trans-silencers as well as their targets reside in single-copy, gene-rich regions that are only sparsely populated with repetitive or transposable elements, features that have been shown to mediate epigenetic activity in other cases [ 18 31 32 ] . Therefore trans-silencing of GFP-COP1 is probably not mediated by transgene flanking sequences [ 27 ] . In contrast, as with other genes cited above, trans-silencing ability is correlated with transgene locus structure, given that the C73 and C97 trans-silencers contain multiple T-DNAs while their targets, E82 and L91, are essentially dimeric and monomeric, respectively.
The structure of the scaffold is entirely consistent with previous 2Lh mapping studies in that the order of six single-copy sequences and the predicted Not I, Pme I, and Sfi I restriction maps of the scaffold (except for four regions corresponding to sequence gaps) are the same as those observed in the corresponding BAC contig and on genomic Southern blots.
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