Words similar to sige

Example sentences for: sige

How can you use “sige” in a sentence? Here are some example sentences to help you improve your vocabulary:

1. 2) A 760-bp fragment containing 526 bp of the 5' region of the M. smegmatis sigE gene and 234 bp of the region upstream was PCR amplified using primer 7206 and primer 7207 (which contains a Hpa I restriction site), digested with Hpa I, and cloned into the Hpa I site of pSEKO.
To differentiate between σ E-dependent and -independent promoters, approximately 40,000 kanamycin-resistant transformants were pooled, and plasmid DNA was isolated and electroporated into M. smegmatis LR222::Δ sigE bacteria, in which the wild type copy of the sigE gene had been disrupted with the gentamicin-resistance gene.
4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene and the lacZ gene.
One transformant was chosen as the M. smegmatis sigE mutant strain and designated LR222Δ sigE (Table 1).
3) The gentamicin-resistance gene ( aacC1 ) was PCR-amplified from pPR27 as a 1270-bp fragment using primer 7204 and primer 7205 (both of which contain Hpa I restriction sites), digested with Hpa I, and cloned into the Hpa I site located between the two fragments of the sigE gene in pSEKO.