Example sentences for: sige

How can you use “sige” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).

  • 4 (Figure 3) was constructed to interrupt the M. smegmatis sigE gene by gene replacement [ 14 ] . pSEKO.

  • In this strain, the M. tuberculosis sigE gene is maintained in a single copy in the genome and is constitutively expressed from the hsp60 promoter.

  • 2 bacteria, which constitutively express the M. tuberculosis sigE gene.

  • 1) A 757-bp fragment containing 246 bp of the 3' end of the M. smegmatis sigE gene and 511 bp of the 5' end of the adjacent ORF1 gene was PCR amplified using primer 7208 (which contains a Hpa I restriction site) and primer 7209 and cloned into the Eco RV blunt-cloning site of pT7Blue to generate pSEKO.


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