Example sentences for: sige

How can you use “sige” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1. 5) The M. tuberculosis sigE gene was PCR amplified from H37Rv genomic DNA using primer 987917 and primer 987916 (which contains a Bam HI site), digested with Bam HI, and cloned into pMV261 digested with Hpa I and Bam HI.

  • 2 bacteria, which constitutively express the M. tuberculosis sigE gene.

  • 4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene and the lacZ gene.

  • Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).

  • In this strain, the M. tuberculosis sigE gene is maintained in a single copy in the genome and is constitutively expressed from the hsp60 promoter.


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