Words similar to sige

Example sentences for: sige

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2 bacteria, which constitutively express the M. tuberculosis sigE gene.
Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).
3) The gentamicin-resistance gene ( aacC1 ) was PCR-amplified from pPR27 as a 1270-bp fragment using primer 7204 and primer 7205 (both of which contain Hpa I restriction sites), digested with Hpa I, and cloned into the Hpa I site located between the two fragments of the sigE gene in pSEKO.
24 white transformants were analyzed by PCR using primers (7997 and 7998) that bind to the sigE gene on either side of the aacC1 gene (Table 2).
One transformant was chosen as the M. smegmatis sigE mutant strain and designated LR222Δ sigE (Table 1).