Words similar to sige
Example sentences for: sige
How can you use “sige” in a sentence? Here are some example sentences to help you improve your vocabulary:
4 (Figure 3) was constructed to interrupt the M. smegmatis sigE gene by gene replacement [ 14 ] . pSEKO.
2 bacteria, which constitutively express the M. tuberculosis sigE gene.
To differentiate between σ E-dependent and -independent promoters, approximately 40,000 kanamycin-resistant transformants were pooled, and plasmid DNA was isolated and electroporated into M. smegmatis LR222::Δ sigE bacteria, in which the wild type copy of the sigE gene had been disrupted with the gentamicin-resistance gene.
Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).
4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene and the lacZ gene.