Words similar to sige

Example sentences for: sige

How can you use “sige” in a sentence? Here are some example sentences to help you improve your vocabulary:

1. 2) A 760-bp fragment containing 526 bp of the 5' region of the M. smegmatis sigE gene and 234 bp of the region upstream was PCR amplified using primer 7206 and primer 7207 (which contains a Hpa I restriction site), digested with Hpa I, and cloned into the Hpa I site of pSEKO.
2. These steps resulted in a 2-bp deletion of the sigE gene.
1) A 757-bp fragment containing 246 bp of the 3' end of the M. smegmatis sigE gene and 511 bp of the 5' end of the adjacent ORF1 gene was PCR amplified using primer 7208 (which contains a Hpa I restriction site) and primer 7209 and cloned into the Eco RV blunt-cloning site of pT7Blue to generate pSEKO.
4 (Figure 3) was constructed to interrupt the M. smegmatis sigE gene by gene replacement [ 14 ] . pSEKO.
Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).