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Recent work in Drosophila tissue culture has shown that endogenous Ptc and Smo proteins are differentially affected by the addition of Hh to the growth medium [ 5 ] . Ptc was destabilized, while Smo accumulated following post-translational modification.
2 is present at much higher concentrations within the cell [ 22 23 ] . This ligand specificity of the NO binding domain of animal SGCs is very similar to bacterial cytochrome c' that likewise binds only NO and CO [ 24 ] . The N-terminal region of the α subunit has been shown to bind certain synthetic pyrazolopyridine ligands, which act as potents NO-independent agonists of cyclase activity [ 25 26 ] .
Comparisons of AEC and DAB on cervical tissues ISH processed with DAKO wide-spectrum probe are shown in Figures 3(normal tissue), 5 (condylomatous lesion); 6 (CIN I); 8 (CIN II); 9 (CIN III); and 10 (squamous cell carcinoma).
In heterologous expression systems, the α 2 -δ subunit was shown to affect the rate of Ca 2+current inactivation and charge movement immobilization [ 33].
Since there was no detectable change in cell size accompanying Siah1a expression measured electrophysiologically (data not shown), the fluorescence measurments were not normalized to total area.