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Example sentences for: shibatae

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While rec12 +was reported to encode a protein of 139 amio acids [ 30 ] , conceptual translation of the surrounding region suggested that rec12 +encoded a larger protein with homology to Spo11 of S. cerevisiae and Top6A of Sulfolobus shibatae [ 17 21 ] . Alignments and splicing sequence profiles [ 31 ] were used to identify four putative introns (Figure 1A).
Meiotically induced, Spo11 (Rec12)-dependent double-strand DNA (dsDNA) breaks have been demonstrated in budding yeast and fission yeast [ 12 13 14 15 ] . Spo11 almost certainly catalyzes these breaks, as it shares high homology with the A subunit of Topoisomerase VI of Sulfolobus shibatae [ 16 17 ] and it becomes linked by a phosphotyrosine linkage to the 5' end of the cleaved DNA [ 18 19 20 21 ] . Spo11 homologs have been identified in a wide range of eukaryotes, and available evidence suggests that initiation of meiotic recombination via Spo11-dependent dsDNA break formation is conserved [ 22 23 24 25 ] . Our results with the fission yeast S. pombe fully support this view (see results and discussion).