Words similar to shaking
Example sentences for: shaking
How can you use “shaking” in a sentence? Here are some example sentences to help you improve your vocabulary:
Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.
Membranes were blocked with Buffer A (Tris-buffered saline (TBS) (Bio-Rad) containing 5.0% skim milk and 0.5% Tween 20 (Sigma)) for 1 h with gentle shaking, then washed with Buffer B (TBS containing 0.1% Tween 20) 3 times for 5 min each.
There is no dissenter in The New New Thing , no East Coast sage shaking his head sadly and warning that it is all going to turn out badly.
The resulting biotinylated adenoviral vectors were incubated at 25°C for 2 h with gentle shaking in polystyrene wells, on which streptavidin is covalently immobilized (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Strip Plates; Pierce Chemical) (5 × 10 6viral particles per well).
Blots were first incubated overnight with gentle shaking at 4°C with rabbit anti-human TRAIL polyclonal antibodies (Cell Sciences, Inc., Norwood, MA) at a concentration of 0.2 μg/ml in Buffer A. Membranes were then washed the following day 3 times for 5 min in Buffer B with gentle shaking, then incubated for 1 h with gentle shaking at room temperature with horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (Amersham Biosciences Corp., Piscataway, NJ) diluted 1:5000 in Buffer A. Reactive proteins were visualized by enhanced chemiluminescence (Amersham, Arlington Heights, IL) following exposure to X-ray film and subsequent film development.
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