Example sentences for: shaker

How can you use “shaker” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After placing the lids on the dishes, the dishes were secured to an orbital tabletop shaker (VWR Scientific Products, Thorofare, New Jersey) and rotated for 20 minutes at 360 RPM.

  • Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg Cdk2 antibody in 300 μl IP buffer (50 mM Tris, pH 7.5; 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, 1% NP-40) and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.

  • The resulting products were resuspended in 50 μl sterile deionized water at room temperature on a rotary shaker for 30 minutes.

  • For example, the Torpedo acetylcholine receptor and the Shaker K +channel protein differ in the fractions of protein glycosylated, the composition of the oligosaccharide chains, and the degree of protein maturation when expressed in Xenopus oocytes as compared with channels in native tissue or other expression systems [ 7 8 ] . Differences in glycosylation patterns can influence the binding of external blocking agents.

  • The membrane was incubated overnight at 4°C on a shaker, washed 4 × 15 minutes with PBS at room temperature (RT), incubated with a horseradish peroxidase-coupled anti-mouse antibody (1:5000, Dianova, Hamburg) for 3 h at and then washed again 4 × 15 minutes with PBS at RT.


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