Words similar to sexta
Example sentences for: sexta
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For slot blot assays, 5 μg of M. sexta or B. mori BBMVs and 40 μl of candidate receptor fractions were blotted onto PVDF membrane and assays was carried out as reported previously [ 27 ] , except for using 1:1000 anti-Bt-R 1 polyclonal antiserum.
BLAST sequence alignments [ 22 ] yielded 81% identity and 96% similarity between this 63 residue stretch and the homologous region of M. sexta APN-1, which also binds Cry1Aa toxin (Jenkins, unpublished observation).
This is not altogether surprising because Cry1Aa binding to Manduca sexta APN has not been found to be modulated by sugar binding [ 8 ] and the B. mori APN sequence is 73.
Recently, cDNA variants of BtR175 have been discovered, showing at least three alleles of the cadherin-like receptor are found in B. mori [ 17 ] . It is likely that glycosylation plays a major role in cadherin-like receptor isoforms as well, as glycosylation has been observed previously for the M. sexta cadherin-like receptor BT-R 1 [ 18 ] .
Recently, using phage display technology, a scFv molecule with short sequence homology to M. sexta and B. mori cadherin-like receptors was shown to bind domain II of Cry1Aa, Cry1Ab, and Cry1Ac toxins [ 20 ] . In the present study, only Cry1Aa shows measurable binding to the purified, native cadherin-like receptor from B. mori . This finding may be the result of purification of a particular receptor variant with Cry1Aa specificity (e.g.