Words similar to serum-supplemented

Example sentences for: serum-supplemented

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These data provide additional evidence that 4-AP exerts neither an anti-proliferative nor a pro-apoptotic influence on serum-supplemented GC cultures, because PCNA expression is a sensitive marker of GC proliferation and apoptosis [ 18 26 27 ] . The lack of 4-AP effect on GC viability is also evident from the flow cytometric analysis; the percentages of cells staining positive for propidium iodide were 8 ± 2% and 7 ± 2% in the presence and absence of drug (n = 4 samples of 10,000 cells/each from 4 GC isolations).
Treatment with 4-AP (2 mM) decreased progesterone accumulation in the media of serum-supplemented GC cultures at 24 h and 48 h post-treatment, in the presence and absence of FSH (Figure 1).
6 mm and a growth area of 1.9 cm 2. The culture media were changed 16 h after plating to fresh serum-supplemented media that contained either vehicle or treatment(s).
The effects of 4-AP in serum-supplemented GC cultures are consistent with previous reports that antagonism of voltage-sensitive K +currents can modulate basal and gonadotropin-stimulated progesterone accumulation in the absence of any effect on cell growth or death.
We concluded that the anti-proliferative effect of 4-AP may contribute to the decreased progesterone accumulation observed in serum-free but not serum-supplemented GC cultures, then focused additional efforts on understanding other mechanisms responsible for 4-AP inhibition of progesterone production.