Words similar to serum-supplemented
Example sentences for: serum-supplemented
How can you use “serum-supplemented” in a sentence? Here are some example sentences to help you improve your vocabulary:
We concluded that the anti-proliferative effect of 4-AP may contribute to the decreased progesterone accumulation observed in serum-free but not serum-supplemented GC cultures, then focused additional efforts on understanding other mechanisms responsible for 4-AP inhibition of progesterone production.
The effects of 4-AP in serum-supplemented GC cultures are consistent with previous reports that antagonism of voltage-sensitive K +currents can modulate basal and gonadotropin-stimulated progesterone accumulation in the absence of any effect on cell growth or death.
6 mm and a growth area of 1.9 cm 2. The culture media were changed 16 h after plating to fresh serum-supplemented media that contained either vehicle or treatment(s).
Our finding that 24 h exposure to 4-AP decreased the number of viable GC in serum-free but not serum-supplemented primary cultures is consistent with the reported effects of 4-AP and other K +channel antagonists on other cell types [ 8 43 44 ] . It has been shown that the concentrations of K +channel antagonists required to inhibit growth of human bladder tumor cells can be 70 times higher in the presence than the absence of serum [ 43 ] . 4-AP has been shown to inhibit the proliferation of human myelobastic leukemia cells by preventing growth factor activation of mitogen activated protein kinase (MAPK) pathways [ 42 ] . This mechanism may be responsible for the anti-proliferative effect of 4-AP manifest in Table 1. It is likely that MAPK pathways are less robust in GC grown in defined serum-free vs. serum-supplemented media; however, validation of this hypothesis would require additional experiments beyond the scope of the present investigation.
Treatment with 4-AP (2 mM) decreased progesterone accumulation in the media of serum-supplemented GC cultures at 24 h and 48 h post-treatment, in the presence and absence of FSH (Figure 1).
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