Words similar to serum-supplemented
Example sentences for: serum-supplemented
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Our finding that 24 h exposure to 4-AP decreased the number of viable GC in serum-free but not serum-supplemented primary cultures is consistent with the reported effects of 4-AP and other K +channel antagonists on other cell types [ 8 43 44 ] . It has been shown that the concentrations of K +channel antagonists required to inhibit growth of human bladder tumor cells can be 70 times higher in the presence than the absence of serum [ 43 ] . 4-AP has been shown to inhibit the proliferation of human myelobastic leukemia cells by preventing growth factor activation of mitogen activated protein kinase (MAPK) pathways [ 42 ] . This mechanism may be responsible for the anti-proliferative effect of 4-AP manifest in Table 1. It is likely that MAPK pathways are less robust in GC grown in defined serum-free vs. serum-supplemented media; however, validation of this hypothesis would require additional experiments beyond the scope of the present investigation.
We concluded that the anti-proliferative effect of 4-AP may contribute to the decreased progesterone accumulation observed in serum-free but not serum-supplemented GC cultures, then focused additional efforts on understanding other mechanisms responsible for 4-AP inhibition of progesterone production.
These data provide additional evidence that 4-AP exerts neither an anti-proliferative nor a pro-apoptotic influence on serum-supplemented GC cultures, because PCNA expression is a sensitive marker of GC proliferation and apoptosis [ 18 26 27 ] . The lack of 4-AP effect on GC viability is also evident from the flow cytometric analysis; the percentages of cells staining positive for propidium iodide were 8 ± 2% and 7 ± 2% in the presence and absence of drug (n = 4 samples of 10,000 cells/each from 4 GC isolations).
Treatment with 4-AP (2 mM) decreased progesterone accumulation in the media of serum-supplemented GC cultures at 24 h and 48 h post-treatment, in the presence and absence of FSH (Figure 1).
Freshly isolated GC were plated in the basic serum-supplemented culture media on collagen-coated 24-well (500 μl/well) or 6-well (2 ml/well) culture dishes, and incubated at 37° C in a humidified atmosphere of 5% CO 2 and air.
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