Example sentences for: semi-nested

How can you use “semi-nested” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DNA was subjected to a semi-nested PCR for VH genes (see supplementary material).

  • By semi-nested PCR, otherwise used for single-cell analysis [ 19], one sole VH-DH-JH rearrangement might be identified.

  • The limit of detection of our semi-nested RT-PCR assay with primers OL26, OL27 and JWA-1b is in the range of 1.3 TCID 50 /filter, yielding a clearly visible 292 bp band.

  • The semi-nested primer pair of OL26 and JWA-1b generates an amplicon of ~292 base pairs from within the original amplicon.

  • Prior to the aerosol experiments, we spiked filters with progressively more dilute viral suspensions to determine the limit of detection of our extraction and semi-nested RT-PCR assay.


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