Words similar to self-splicing

Example sentences for: self-splicing

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However, four of the constructs, Th1P6, Th2P6, Th3P6, and Th1P5, showed good activity (>10% of wild-type) and increased self-splicing in the presence of theophylline.
The generalized group II intron secondary structure consists of six helical domains emerging from a central wheel [ 1 2 ] . Some group II introns are capable of self-splicing; however for efficient splicing in vivo , the intron-encoded ORF or host-encoded splicing factors are required [ 3 ] . Mobile group II introns encode a reverse transcriptase/maturase, with the coding region generally located in domain IV.
In this regard, it is notable that some natural Group I introns have proven to be highly dependent upon protein cofactors, [ 35 ] and that Group I introns have been engineered whose tertiary structure and activity is dependent upon a peptide [ 36 ] . Such applications could also be melded with other innovations dependent upon the Group I self-splicing intron, such as the introduction of a trans-splicing intron that can potentially repair or modulate the expression of a given mRNA.
However, the Group I self-splicing intron is a much more complicated ribozyme than either the hammerhead or the L1 ligase; for example, the tertiary structure of the Group I intron is established by a complicated folding pathway.
The self-splicing activities of the Group I aptazymes were examined in vitro using a standard splicing assay (Table 1).