Words similar to self-splicing

Example sentences for: self-splicing

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Each of the successful aptazyme constructs was subsequently cloned into an interrupted thymidylate synthase gene in place of the parental td self-splicing intron.
Despite the chemical and physical differences between the E. coli cytoplasm and the environment in which the T. acidophilum intein is functioning in vivo, we found no indications that self-splicing of the intein was inefficient in E. coli.
However, four of the constructs, Th1P6, Th2P6, Th3P6, and Th1P5, showed good activity (>10% of wild-type) and increased self-splicing in the presence of theophylline.
In this regard, it is notable that some natural Group I introns have proven to be highly dependent upon protein cofactors, [ 35 ] and that Group I introns have been engineered whose tertiary structure and activity is dependent upon a peptide [ 36 ] . Such applications could also be melded with other innovations dependent upon the Group I self-splicing intron, such as the introduction of a trans-splicing intron that can potentially repair or modulate the expression of a given mRNA.
The self-splicing activities of the Group I aptazymes were examined in vitro using a standard splicing assay (Table 1).