Words similar to seb

Example sentences for: seb

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Other investigators have shown (in mouse systems) that commitment to Th1 or Th2 cytokine production by naïve T cells requires prior cell proliferation [ 24 ] ; and reversal of Th1 or Th2-committed cells requires at least several days of proliferation [ 25 ] . When normal human PBMC were stimulated for 7 days with SEB, the presence of 5A6 anti-CD81 mAb resulted in a modest increase in IL-4-producing T cells, when those cells were restimulated with PMA and ionomycin (Figure 4A).
For experiments examining cytokine production and CD69 induction, a "response region" was defined for CD69 +cytokine +cells using a positive control such as SEB stimulation, on a CD4-gated dot plot.
T cells (treated or not) were recombined with non-T cells (treated or not) for stimulation with SEB as described below.
For experiments examining proliferation, PBMC were incubated with SEB for 48 h at 37°C, with addition of 60 μM BrdU (Sigma) and 10 μg/ml Brefeldin A for the last 6 h.
Similar results were seen when PBMC were stimulated with plate-bound anti-CD3 mAb, rather than SEB, for 7 days in the presence of 5A6 anti-CD81 mAb (Figure 4B).