Example sentences for: seap

How can you use “seap” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Eighty-four luciferase transfected clones and 113 SEAP transfected clones were transferred from the 96 well plates and duplicate wells of each were treated with either DMSO or 15 μM GS-E in DMSO for 24 hours.

  • Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).

  • Importantly, we have demonstrated similar kinetics for 2 different cancer cell lines with 2 different reporters (F-luc and SEAP) thus validating the generality of the observed results.

  • Assays for the appropriate reporter in each cell pool showed induction ratios of 17-fold for the luciferase transfected cells and 22-fold for the SEAP transfected cells.

  • We have demonstrated the general applicability of this system by demonstrating its use in two different mouse tumor cell lines, integration of the RHeoSwitch DNA components at multiple chromosomal locations, as well as with 2 different gene reporters, luciferase, and SEAP.


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