Words similar to seap

Example sentences for: seap

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These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.
Eighty-four luciferase transfected clones and 113 SEAP transfected clones were transferred from the 96 well plates and duplicate wells of each were treated with either DMSO or 15 μM GS-E in DMSO for 24 hours.
The inducible reporter gene plasmids, pG5-luc and pGAL4-SEAP, were transfected into the Panc02+RHeoSwitch-3 and -4 cells and stable transfectants were isolated as they were into the MBT-2+RHeoSwitch cells.
Assays were performed using the chemiluminescent SEAP assay procedure as described by the manufacturer in the Great EscAPe SEAP Assay system (Clontech).
Multiple assays in triplicate on the GAL4-SEAP transfected clones gave induction levels ranging from 118- to 551-fold for the best five Panc02+RHeoSwitch-3 clones and from 52- to 486-fold for the best twelve Panc02+RHeoSwitch-4 clones.