Words similar to seap
Example sentences for: seap
How can you use “seap” in a sentence? Here are some example sentences to help you improve your vocabulary:
Four independent Panc02+RHeoSwitch+SEAP clones also induced expression by 12 hours but the level of SEAP activity continued to increase for 72 hours, possibly due to the accumulation of the enzyme in the cell culture media over the entire treatment time.
These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).
This verified the presence of the GS-E inducible reporter genes (luciferase or SEAP) in the cloned pool of cells.
The inducible reporter gene plasmids, pG5-luc and pGAL4-SEAP, were transfected into the Panc02+RHeoSwitch-3 and -4 cells and stable transfectants were isolated as they were into the MBT-2+RHeoSwitch cells.