Words similar to seap
Example sentences for: seap
How can you use “seap” in a sentence? Here are some example sentences to help you improve your vocabulary:
Multiple assays in triplicate on the GAL4-SEAP transfected clones gave induction levels ranging from 118- to 551-fold for the best five Panc02+RHeoSwitch-3 clones and from 52- to 486-fold for the best twelve Panc02+RHeoSwitch-4 clones.
These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.
Assays were performed using the chemiluminescent SEAP assay procedure as described by the manufacturer in the Great EscAPe SEAP Assay system (Clontech).
Four independent Panc02+RHeoSwitch+SEAP clones also induced expression by 12 hours but the level of SEAP activity continued to increase for 72 hours, possibly due to the accumulation of the enzyme in the cell culture media over the entire treatment time.
This verified the presence of the GS-E inducible reporter genes (luciferase or SEAP) in the cloned pool of cells.
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