Example sentences for: seap

How can you use “seap” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).

  • These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.

  • Eighty-four luciferase transfected clones and 113 SEAP transfected clones were transferred from the 96 well plates and duplicate wells of each were treated with either DMSO or 15 μM GS-E in DMSO for 24 hours.

  • The inducible reporter gene plasmids, pG5-luc and pGAL4-SEAP, were transfected into the Panc02+RHeoSwitch-3 and -4 cells and stable transfectants were isolated as they were into the MBT-2+RHeoSwitch cells.

  • Multiple Zeocin resistant cell clones from the four transfections (36 Panc02+RHeoSwitch-3+luc, 75 Panc02+RHeoSwitch-4+luc, 8 Panc02+RHeoSwitch-3+GAL4-SEAP, 98 Panc02+RHeoSwitch-4+SEAP) were plated in duplicate and treated with DMSO or 15 μM GS-E.


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