Example sentences for: scraped

How can you use “scraped” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Transfected cells were scraped from the dishes, washed twice with Dulbecco's PBS (New Life Technologies, NY), then resuspended in 1.0 ml ice cold PBS/100 mm dish.

  • The scraped cells were centrifuged at 2000 × g for 5 min at 4°C, the supernatant was discarded, and the pellet was stored at -80°C until assayed.

  • Cell-fractionation was carried out mostly as previously described [ 23 ] . For isolation of nuclei and nuclear envelopes, HeLa cells from 10 15-cm culture dishes were washed twice with ice-cold PBS and scraped off in PBS.

  • The adherent cells in the well were washed three times with 1.0 ml of PBS, and the cells overlaid with 400 μl of lysis buffer (1% Triton X-100 in pH 7.5, 20 mM HEPES buffer with 0.1 mM EDTA), scraped, and collected into a glass vial for tocopherol analysis by HPLC, and for protein determination by the BCA protein assay (Pierce, Rockford, IL).

  • When the cells were extracted with Triton X-100, NP-40 or saponin after relatively weak fixation with formaldehyde, the cytoskeleton apparently was lost, fragmented, or scraped off depending on the concentration of detergents and the period of incubation (Table 2).


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast